Document Detail


Analysis of animal and plant selenometabolites in roots of a selenium accumulator, Brassica rapa var. peruviridis, by speciation.
MedLine Citation:
PMID:  23348393     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Many studies have examined the metabolic pathway of selenium (Se) compounds in Se-accumulating plants (hereafter "Se accumulators") when the plants are exposed to inorganic Se, such as selenite and selenate. However, if we were to consider Se circulation in the biosphere, the metabolism of organic Se, in particular, selenometabolites of animals and plants, in plants should be elucidated. In this study, Brassica rapa var. peruviridis, a known Se accumulator, was hydroponically cultivated and then exposed to selenometabolites of animals and plants, such as methyl-2-acetamido-2-deoxy-1-seleno-β-d-galactopyranoside (selenosugar, SeSug), trimethylselenonium (TMSe), selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys). Then, the metabolic pathway of the organic Se compounds/selenometabolites in B. rapa var. peruviridis was investigated by speciation analysis. Two selenometabolites were detected in the roots when the plant was exposed to SeMet, MeSeCys, and SeSug. They were assigned to S-(methylseleno)-glutathione and MeSeCys using electrospray tandem mass spectrometry (ESI-MS-MS) and HPLC-inductively coupled plasma mass spectrometry (ICP-MS). Contrary to SeMet, MeSeCys, and SeSug, TMSe was not metabolized even if it was more efficiently incorporated into the roots than the other Se compounds. The identified metabolites enabled us to propose a metabolic pathway for the organic Se metabolites except TMSe in the plant roots: a monomethylseleno moiety (CH3Se-) commonly existing in SeMet, MeSeCys, and SeSug was cleaved off and conjugated with GSH, and then the CH3Se group was transferred to O-acetylserine to form MeSeCys.
Authors:
Yasumitsu Ogra; Ayane Katayama; Yurie Ogihara; Ayako Yawata; Yasumi Anan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-25
Journal Detail:
Title:  Metallomics : integrated biometal science     Volume:  5     ISSN:  1756-591X     ISO Abbreviation:  Metallomics     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-05-02     Completed Date:  2013-11-18     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  101478346     Medline TA:  Metallomics     Country:  England    
Other Details:
Languages:  eng     Pagination:  429-36     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Brassica rapa / drug effects,  metabolism*
Chromatography, High Pressure Liquid
Metabolic Networks and Pathways / drug effects
Organoselenium Compounds / metabolism*,  pharmacology
Plant Extracts / chemistry
Plant Roots / drug effects,  metabolism*
Selenium / metabolism*
Selenium Compounds / pharmacology
Selenocysteine / analogs & derivatives,  pharmacology
Selenomethionine / pharmacology
Spectrometry, Mass, Electrospray Ionization
Water / chemistry
Chemical
Reg. No./Substance:
0/Organoselenium Compounds; 0/Plant Extracts; 0/Selenium Compounds; 059QF0KO0R/Water; 0CH9049VIS/Selenocysteine; 2574-71-2/selenomethylselenocysteine; 25930-79-4/trimethylselenonium; 964MRK2PEL/Selenomethionine; H6241UJ22B/Selenium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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