Document Detail

Analysis of anaerobic fluorescence decay in Scenedesmus obliquus.
MedLine Citation:
PMID:  1125286     Owner:  NLM     Status:  MEDLINE    
With reduction of System II acceptors during dark anaerobic adaptation in Scenedesmus obliquus fluorescence yield rises to a maximum value in two distinct transitions. Subsequent illumination results in a decay of fluorescence yield with the following characteristics: 1. In low intensity light it is independent of temperature and is an expression of light reaction I. 2. In high intestity light it reflects the dark limiting step in the reoxidation mechanism of System II primary acceptors. 3. There is strong inhibition by agents known to block electron transport between the two systems. 4. At light limiting conditions decay kinetics include an initial delay phase and thereafter close to second order behaviour. 5. Following a single brief saturating flash a maximum of 80% quenching is restored and a second flash yields approx. 95% restoration. Comparison with the fluorescence rise in the presence of 3-(3,4-dichlorophenyl)-1, 1-dimethylurea suggests that the decay reflects transfer of one positive charge from System I to the primary System II acceptor with the intermediary carrier pool remaining reduced.
W Schreiber UVidaver
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  387     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1975 Apr 
Date Detail:
Created Date:  1975-07-28     Completed Date:  1975-07-28     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  37-51     Citation Subset:  IM    
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MeSH Terms
Algae, Green / metabolism*
Diuron / pharmacology
Electron Transport
Methylurea Compounds
Quinones / pharmacology
Spectrometry, Fluorescence
Time Factors
Reg. No./Substance:
0/Methylurea Compounds; 0/Quinones; 330-54-1/Diuron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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