Document Detail


Analysis of amino acids and biogenic amines in biological tissues as their o-phthalaldehyde/ethanethiol/fluorenylmethyl chloroformate derivatives by high-performance liquid chromatography. A deproteinization study.
MedLine Citation:
PMID:  17145062     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The extraction of ornithine, lysine, putrescine, cadaverine, 1,7-diaminoheptane, spermidine and spermine from biological tissues was optimized for HPLC quantitation as their o-phthalaldehyde/ethanethiol/fluorenylmethyl chloroformate (OPA/ET/FMOC) derivatives. In applying perchloric acid deproteinization two approaches have been followed: (i) deproteinization with subsequent neutralization by potassium hydroxide and lyophilization, and (ii) deproteinization without neutralization and lyophilization. Neutralization and lyophilization resulted in the loss of free biogenic amines. HPLC analysis of ornithine (Orn), lysine (Lys), putrescine (Put), cadaverine (Cad), 1,7-diaminoheptane (Dah), spermidine (Spd) and spermine (Spm) content of biological tissues as their OPA/ET/FMOC derivatives was performed in the supernatant of perchloric acid-deproteinized samples (model solutions and tissues) with an average reproducibility of < or =2.6% relative standard deviation (RSD), including recovery of sample treatment and chromatography.
Authors:
A Korös; R Hanczkó; A Jámbor; Y Qian; A Perl; I Molnár-Perl
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-12-04
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1149     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-30     Completed Date:  2007-07-27     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  46-55     Citation Subset:  IM    
Affiliation:
Institute of Chemistry, Department of Analytical Chemistry, L. Eötvös University, Budapest, Hungary.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*
Animals
Biogenic Amines / analysis*
Chromatography, High Pressure Liquid / methods*
Fluorenes / chemistry*
Mice
Reference Standards
Reproducibility of Results
o-Phthalaldehyde / chemistry*
Grant Support
ID/Acronym/Agency:
R01 DK49221/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Biogenic Amines; 0/Fluorenes; 28920-43-6/1-(9-fluorenyl)methyl chloroformate; 643-79-8/o-Phthalaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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