Document Detail

Analysis of amikacin-resistant Pseudomonas aeruginosa developing in patients receiving amikacin.
MedLine Citation:
PMID:  2493230     Owner:  NLM     Status:  MEDLINE    
During a 36-month period, 28 patients treated for infections due to amikacin-susceptible Pseudomonas aeruginosa subsequently developed infections or colonization with amikacin-resistant P aeruginosa at the same site. Eleven amikacin-susceptible/-resistant pairs of isolates were analyzed for aminoglycoside-inactivating enzymes, plasmid profiles, cellular proteins, outer membrane proteins (OMPs), lipopolysaccharide (LPS) profiles, and amikacin uptake. While clearly distinct from isolates of other patients, sensitive and resistant isolates from the same patients were indistinguishable in plasmid profile, LPS profiles, and OMPs. These results suggest that the resistant P aeruginosa isolates were derived from the sensitive isolates. None of the resistant isolates produced enzymes known to inactivate amikacin. In nine of 11 resistant isolates tested, transport of amikacin into P aeruginosa was reduced. A major mechanism of in vivo development of amikacin resistance in P aeruginosa is alteration in permeability to amikacin, but the aquisition of plasmids or changes in OMPs or LPS profile may not account for this phenomenon.
J Maloney; D Rimland; D S Stephens; P Terry; A M Whitney
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Archives of internal medicine     Volume:  149     ISSN:  0003-9926     ISO Abbreviation:  Arch. Intern. Med.     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-04-06     Completed Date:  1989-04-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372440     Medline TA:  Arch Intern Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  630-4     Citation Subset:  AIM; IM    
Department of Medicine, Emory University School of Medicine, Atlanta, GA.
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MeSH Terms
Amikacin / therapeutic use*
Bacterial Outer Membrane Proteins / analysis
Bacterial Proteins / analysis
Drug Resistance, Microbial
Electrophoresis, Polyacrylamide Gel
Lipopolysaccharides / analysis
Microbial Sensitivity Tests
Pseudomonas Infections / drug therapy*
Pseudomonas aeruginosa / drug effects*,  isolation & purification
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Lipopolysaccharides; 37517-28-5/Amikacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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