Document Detail


Analysis of adenovirus infections in synchronized cells.
MedLine Citation:
PMID:  17656777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adenoviruses (Ads) are small DNA tumor viruses that have played a pivotal role in understanding eukaryotic cell biology and viral oncogenesis. Among other cellular pathways, Ad usurps cell cycle progression following infection. Likewise, progression of the viral infection is influenced by the host cell cycle. We describe here methods developed for synchronizing dividing cell populations and for analysis of cell cycle synchrony by flow cytometry. Furthermore, three methods used to evaluate the outcome of Ad infection in synchronized cell populations are described. These include two assays for infectious centers and an assay for analyzing production of progeny virus by transmission electron microscopy. These methods have been used to demonstrate that Ads that fail to direct synthesis of the E1B 55-kDa or E4orf6 proteins replicate most effectively upon infecting cells in S phase.
Authors:
David A Ornelles; Robin N Broughton-Shepard; Felicia D Goodrum
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in molecular medicine     Volume:  131     ISSN:  1543-1894     ISO Abbreviation:  Methods Mol. Med.     Publication Date:  2007  
Date Detail:
Created Date:  2007-07-27     Completed Date:  2007-09-18     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  101123138     Medline TA:  Methods Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  83-101     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics,  physiology*,  ultrastructure
Culture Media, Conditioned
DNA, Viral / genetics
Flow Cytometry
Hela Cells / virology
Humans
Microscopy, Electron, Transmission
Grant Support
ID/Acronym/Agency:
AI 35589/AI/NIAID NIH HHS; CA 12197/CA/NCI NIH HHS; CA 77342/CA/NCI NIH HHS; CA 77342S1/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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