Document Detail

Analysis of aclarubicin-induced cell death in human fibroblasts.
MedLine Citation:
PMID:  17498978     Owner:  NLM     Status:  MEDLINE    
In the present study we investigated the mode of cell death induced by aclarubicin (ACL) in trisomic (BB) and normal (S-2) human fibroblasts. Cells were incubated with ACL for 2h and then cultured in drug-free medium for up to 96h. Using fluorescence microscopy, agarose gel electrophoresis and comet assay we demonstrate that ACL induced time-dependent morphological and biochemical changes in both cell types. The population of apoptotic cells, analysed by acridine orange and ethidium bromide nuclear staining reached its maximum at 24-48h. Prolonged post-treatment time progressively increased the level of necrotic cells. At 24-48h time points we also observed a significant increase in caspase-3 activity, oligonucleosomal DNA fragmentation and DNA strand breaks. Cotreatment of cells with the specific caspase-3 inhibitor Ac-DEVD-CHO partly reduced the extent of apoptosis and necrosis and DNA degradation. In conclusion, trisomic and normal fibroblasts demonstrate similar response to aclarubicin treatment. Drug induced the apoptotic and necrotic pathway of cell death that was mediated by caspase-3.
K Kania; K Matławska-Wasowska; R Osiecka; Z Jóźwiak
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Publication Detail:
Type:  Journal Article     Date:  2007-03-28
Journal Detail:
Title:  Cell biology international     Volume:  31     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-06-04     Completed Date:  2007-08-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  1049-56     Citation Subset:  IM    
Department of Thermobiology, Institute of Biophysics, University of Łódź, Banacha 12/16 Street, 90-237 Łódź, Poland.
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MeSH Terms
Aclarubicin / pharmacology*
Antineoplastic Agents / pharmacology
Caspase 3 / metabolism
Cell Death / drug effects
Cell Proliferation / drug effects
Comet Assay
DNA Fragmentation / drug effects
Down Syndrome / pathology
Enzyme Activation / drug effects
Fibroblasts / cytology*,  drug effects*
Nucleosomes / drug effects
Reg. No./Substance:
0/Antineoplastic Agents; 0/Nucleosomes; 57576-44-0/Aclarubicin; EC 3.4.22.-/Caspase 3

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