| Analysis of TNT (2,4,6-trinitrotoluene)-inducible cellular responses and stress shock proteome in Stenotrophomonas sp. OK-5. | |
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MedLine Citation:
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PMID: 15486709 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, the cellular responses of Stenotrophomonas sp. OK-5 to explosive 2,4,6-trinitrotoluene (TNT) have been extensively analyzed. The stress shock proteins, which might contribute to enhancing cellular resistance to TNT-mediated toxicity, were induced at different concentrations of TNT used as a substrate for cell culture of Stenotrophomonas sp. OK-5 capable of utilizing TNT. Proteomic analysis for 2-DE of soluble protein fractions from the culture of OK-5 exposed to TNT demonstrated approximately 300 spots on the silver-stained gel ranging from pH 3 to pH 10. Among them, 10 spots significantly induced and expressed in response to TNT were selected and analyzed. As the result of internal amino acid sequencing with ESI-Q TOF mass spectrometry, TNT-mediated stress shock proteins such as DnaK, OmpW, and OsmC were identified and characterized. Survival of strain OK-5 was periodically monitored in the presence of different concentrations of TNT along with the production of the stress shock proteins. Cells of strain OK-5 pre-exposed to TNT had in improved survival tolerance. Analysis of total cellular fatty acids in strain OK-5 suggested that several saturated or unsaturated fatty acids might increase or decrease under TNT-mediated stress condition. Scanning electron microscopy of cells treated with 0.8 mM TNT for 12 h revealed irregular rod shapes with wrinkled surfaces. |
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Authors:
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Eun-Mi Ho; Hyo-Won Chang; Seung-Il Kim; Hyung-Yeel Kahng; Kye-Heon Oh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Current microbiology Volume: 49 ISSN: 0343-8651 ISO Abbreviation: Curr. Microbiol. Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-10-15 Completed Date: 2004-12-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7808448 Medline TA: Curr Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 346-52 Citation Subset: IM |
Affiliation:
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Department of Life Science, Soonchunhyang University, Asan, P.O. Box 97, Chung-Nam, 336-600, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Bacterial Proteins / genetics, isolation & purification, metabolism Biodegradation, Environmental Electrophoresis, Gel, Two-Dimensional Environmental Pollutants / metabolism, pharmacology Fatty Acids / metabolism Microscopy, Electron, Scanning Proteome / drug effects, isolation & purification Stenotrophomonas / drug effects*, genetics, metabolism*, ultrastructure Trinitrotoluene / metabolism, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Environmental Pollutants; 0/Fatty Acids; 0/Proteome; 118-96-7/Trinitrotoluene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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