| Analysis of single nucleic acid molecules with protein nanopores. | |
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MedLine Citation:
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PMID: 20627172 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe the methods used in our laboratory for the analysis of single nucleic acid molecules with protein nanopores. The technical section is preceded by a review of the variety of experiments that can be done with protein nanopores. The end goal of much of this work is single-molecule DNA sequencing, although sequencing is not discussed explicitly here. The technical section covers the equipment required for nucleic acid analysis, the preparation and storage of the necessary materials, and aspects of signal processing and data analysis. |
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Authors:
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Giovanni Maglia; Andrew J Heron; David Stoddart; Deanpen Japrung; Hagan Bayley |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in enzymology Volume: 475 ISSN: 1557-7988 ISO Abbreviation: Meth. Enzymol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-07-14 Completed Date: 2010-10-15 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 0212271 Medline TA: Methods Enzymol Country: United States |
Other Details:
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Languages: eng Pagination: 591-623 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Chemistry, University of Oxford, Oxford, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Nanotechnology* Nucleic Acids / chemistry* Porosity Proteins / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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R01 HG003709-06/HG/NHGRI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nucleic Acids; 0/Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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