Document Detail


Analysis of Schizosaccharomyces pombe mitochondrial DNA replication by two dimensional gel electrophoresis.
MedLine Citation:
PMID:  7924618     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The entire mitochondrial genome of Schizosaccharomyces pombe ura4-294h- was analyzed by the 2D pulsed field gel electrophoresis technique developed by Brewer and Fangman. The genome consists of multimers with an average size of 100 kb and analysis of the overlapping restriction fragments of the complete mitochondrial DNA (mtDNA) genome resulted in simple Y 2D gel patterns. Large single-stranded DNA molecules or double-stranded DNA molecules containing large or numerous single-stranded regions were found in the S. pombe mtDNA preparation. The replication of mtDNA monomers was found to occur in either direction. On the basis of these results, a replication mechanism for S. pombe mtDNA that is most consistent with a rolling circle model is suggested.
Authors:
Z Han; C Stachow
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chromosoma     Volume:  103     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-11-10     Completed Date:  1994-11-10     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  162-70     Citation Subset:  IM    
Affiliation:
Department of Biology, Boston College, Chestnut Hill, MA 02167.
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MeSH Terms
Descriptor/Qualifier:
DNA Replication / physiology*
DNA, Fungal / analysis,  biosynthesis*,  chemistry
DNA, Mitochondrial / analysis,  biosynthesis*,  chemistry
DNA, Single-Stranded / analysis,  chemistry
Electrophoresis, Gel, Two-Dimensional
Models, Genetic
Nucleic Acid Conformation
Restriction Mapping
Schizosaccharomyces / genetics*
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/DNA, Mitochondrial; 0/DNA, Single-Stranded

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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