Document Detail


Analysis of a SNP linked to lactase persistence: An exercise for teaching molecular biology techniques to undergraduates.
MedLine Citation:
PMID:  21445905     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Recent experimental evidence indicates that the ability of adults to tolerate milk, cheese, and other lactose-containing dairy products is an autosomal dominant trait that co-evolved with dairy farming in Central Europe about 7,500 years ago. Among persons of European descent, this trait is strongly associated with a C to T substitution at a polymorphic site 13,910 bp upstream of the lactase gene. This mutation results in the persistent expression of lactase into adulthood enabling individuals carrying a T(-13,910) allele to digest lactose as adults. In this report, we describe a laboratory exercise for an undergraduate molecular biology course in which students determine their own genotype at the -13,910 polymorphic site and correlate this with their ability to tolerate dairy products. The exercise is used as a tool to teach basic molecular biology procedures such as agarose gel electrophoresis, PCR1, and DNA sequencing. Students are actively engaged in the learning process, not only by analyzing their own DNA but also by applying their knowledge and skills to answer an authentic question. The exercise is also integrated with lecture material on the control of gene expression at the transcriptional level, in particular, how transcription factors can influence the activity of a promoter by binding to cis-acting DNA regulatory elements located within the proximal promoter of a gene or distant enhancer regions. Biochemistry and Molecular Biology Education Vol. 39, No. 2, pp. 133-140, 2011.
Authors:
Patrick J Schultheis; Bethany V Bowling
Related Documents :
21486805 - Striated muscle activator of rho signalling (stars) is a pgc-1α/oestrogen-related recep...
1927955 - Effects of exercise on left ventricular diastolic performance in trained athletes.
2830325 - Postural exercise abnormalities in symptomatic patients with mitral valve prolapse.
18621855 - The impact of endurance exercise training on left ventricular systolic mechanics.
12663285 - Response of whole-body protein and urea turnover to exercise differs between patients w...
2719145 - Ventromedial hypothalamic regulation of hormonal and metabolic responses to exercise.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology     Volume:  39     ISSN:  1539-3429     ISO Abbreviation:  Biochem Mol Biol Educ     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100970605     Medline TA:  Biochem Mol Biol Educ     Country:  United States    
Other Details:
Languages:  eng     Pagination:  133-40     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Wiley Periodicals, Inc.
Affiliation:
Department of Biological Sciences, Northern Kentucky University, Highland Heights, Kentucky 41099. schultheisp@nku.edu.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Fluorescence-PCR assays and isolation of luminescent bacterial clones using an automated plate reade...
Next Document:  Purification and analysis of colorful hypothetical open reading frames: An inexpensive gateway labor...