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Analysis of Rutin in the Extract and Gel of Viola tricolor.
MedLine Citation:
PMID:  23035206     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Heartsease, also known as wild pansy (Viola tricolor L.), contains considerable amounts of polyphenols: 109.32 ± 1.29 mg of Gallic acid equivalent/g of extract. This study investigates their phytoconstituents and antioxidant capacity and validates a method for the quantification of rutin in the crude extract of the flowers of V. tricolor and in the extract incorporated in gel. Much better antioxidant capacity was found for the extract [inhibition concentration (IC(50)) of 16.00 ± 0.78 µg/mL] than the standard ascorbic acid (IC(50) of 16.57 ± 0.95 µg/mL); these excellent results may be attributable to the amounts of polyphenols, flavonoids and condensed tannins. The high-performance liquid chromatography method for the quantification of rutin in the extract and gel was linear, sensitive, precise, specific, accurate and robust. This validated method can be used to control the quality of the extract and the gel.
Authors:
Mariana Piana; Marina Zadra; Thiele Faccim de Brum; Aline Augusti Boligon; Adiene Fernandes Kieling Gonçalves; Ritiel Corrêa da Cruz; Robson Borba de Freitas; Gizele Scotti do Canto; Margareth Linde Athayde
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-3
Journal Detail:
Title:  Journal of chromatographic science     Volume:  -     ISSN:  1945-239X     ISO Abbreviation:  J Chromatogr Sci     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Build 26, room 1115, Santa Maria, RS 97105-900, Brazil.
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