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Analysis of RNA localization and metabolism in single live bacterial cells: achievements and challenges.
MedLine Citation:
PMID:  21453445     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Studies of gene expression at the single cell level in live bacterial cells represent a new and fertile area of research providing real-time, spatially-specified information that cannot be obtained by techniques relying on large cell populations. Before recently, most single-cell studies have been concerned with gene expression at the protein level and explored the spatio-temporal localization and dynamics of different bacterial proteins. However, to fully understand the complex process of gene expression, it is necessary to visualize and quantify RNA molecules in the cellular environment. The first studies analyzing the kinetics of RNA transcription and the distribution of RNA in single bacterial cells in real time have recently been reported. Here, I discuss the methods allowing RNA detection in living bacterial cells, the results on RNA kinetics and RNA localization, and the challenges for future research in this area.
Authors:
Natalia E Broude
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-1
Journal Detail:
Title:  Molecular microbiology     Volume:  -     ISSN:  1365-2958     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
Affiliation:
Department of Biomedical Engineering, Boston University, 36 Cummington St., Boston, MA 02215.
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