| Analysis of Photoreceptor Rod Outer Segment Phagocytosis by RPE Cells In Situ. | |
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MedLine Citation:
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PMID: 23150373 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Counting rhodopsin-positive phagosomes residing in the retinal pigment epithelium (RPE) in the eye at different times of day allows a quantitative assessment of engulfment and digestion phases of diurnal RPE phagocytosis, which efficiently clears shed photoreceptor outer segment fragments (POS) from the neural retina. Comparing such activities among age- and background-matched experimental wild-type and mutant mice or rats serves to identify roles for specific proteins in the phagocytic process. Here, we describe experimental procedures for mouse eye harvest, embedding, sectioning, immunofluorescence labeling of rod POS phagosomes in RPE cells in sagittal eye sections, imaging of POS phagosomes in the RPE by laser scanning confocal microscopy, and POS quantification. |
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Authors:
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Saumil Sethna; Silvia C Finnemann |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 935 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: - |
Other Details:
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Languages: ENG Pagination: 245-254 Citation Subset: - |
Affiliation:
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Department of Biological Sciences, Fordham University, Bronx, NY, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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