Document Detail


Analysis of photoreceptor outer segment phagocytosis by RPE cells in culture.
MedLine Citation:
PMID:  23150376     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Retinal pigment epithelial (RPE) cells are among the most actively phagocytic cells in nature. Primary RPE and stable RPE cell lines provide experimental model systems that possess the same phagocytic machinery as RPE in situ. Upon experimental challenge with isolated photoreceptor outer segment fragments (POS), these cells promptly and efficiently recognize, bind, internalize, and digest POS. Here, we describe experimental procedures to isolate POS from porcine eyes and to feed POS to RPE cells in culture. Furthermore, we describe three different and complementary methods to quantify total POS uptake by RPE cells and to discriminate surface-bound from engulfed POS.
Authors:
Yingyu Mao; Silvia C Finnemann
Related Documents :
25119266 - 1. normal development of testicular structures: from the bipotential gonad to the fetal...
23062626 - Reprogramming and development in nuclear transfer embryos and in interspecific systems.
23117856 - Erythropoietin receptor expression and its relationship with trastuzumab response and r...
23682426 - Mechanisms involved in the cytotoxic effects of berberine on human colon cancer hct-8 c...
8196496 - Corticotropin-releasing factor is secreted in the be(2)-c neuroblastoma cell and is res...
10671316 - Probing the function of connexin channels in primary tissues.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  935     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-15     Completed Date:  2013-04-17     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  285-95     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / methods
Cell Separation / methods*
Cells, Cultured
Immunoblotting / methods
Microscopy, Fluorescence / methods
Opsins / analysis
Phagocytosis*
Retinal Photoreceptor Cell Outer Segment / ultrastructure*
Retinal Pigment Epithelium / cytology*
Swine
Grant Support
ID/Acronym/Agency:
EY013295/EY/NEI NIH HHS; R01 EY013295/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Opsins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Functional Analysis of Retinal Microglia and Their Effects on Progenitors.
Next Document:  Ca(2+)-Imaging Techniques to Analyze Ca(2+) Signaling in Cells and to Monitor Neuronal Activity in t...