Document Detail

Analysis of photoreceptor outer segment phagocytosis by RPE cells in culture.
MedLine Citation:
PMID:  23150376     Owner:  NLM     Status:  MEDLINE    
Retinal pigment epithelial (RPE) cells are among the most actively phagocytic cells in nature. Primary RPE and stable RPE cell lines provide experimental model systems that possess the same phagocytic machinery as RPE in situ. Upon experimental challenge with isolated photoreceptor outer segment fragments (POS), these cells promptly and efficiently recognize, bind, internalize, and digest POS. Here, we describe experimental procedures to isolate POS from porcine eyes and to feed POS to RPE cells in culture. Furthermore, we describe three different and complementary methods to quantify total POS uptake by RPE cells and to discriminate surface-bound from engulfed POS.
Yingyu Mao; Silvia C Finnemann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  935     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-15     Completed Date:  2013-04-17     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  285-95     Citation Subset:  IM    
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MeSH Terms
Cell Culture Techniques / methods
Cell Separation / methods*
Cells, Cultured
Immunoblotting / methods
Microscopy, Fluorescence / methods
Opsins / analysis
Retinal Photoreceptor Cell Outer Segment / ultrastructure*
Retinal Pigment Epithelium / cytology*
Grant Support
Reg. No./Substance:

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