Document Detail

Analysis of phosphorothionate pesticides using a chloroperoxidase pretreatment and acetylcholinesterase biosensor detection.
MedLine Citation:
PMID:  20614938     Owner:  NLM     Status:  MEDLINE    
Acetylcholinesterase (AChE) is responsible for the hydrolysis of acetylcholine in the nervous system. It is inhibited by organophosphate and carbamate pesticides. However, this enzyme is only slightly inhibited by organophosphorothionates, which makes the detection of these pesticides analytically very difficult. A new enzymatic method for the activation and detection of phosphorothionates was developed with the capability to be used directly in food samples without the need of laborious solvent extraction steps. Chloroperoxidase (CPO) from Caldariomyces fumago was combined with tert-butyl hydroperoxide and two halides. Chlorpyrifos and triazophos were completely oxidized. Fenitrothion, methidathion and parathion methyl showed conversion rates between 54 and 61%. Furthermore, the oxidized solution was submitted to an AChE biosensor assay. Chlorpyrifos spiked in organic orange juice was oxidized, where its oxon product was detected in concentrations down to 5 microg/L (final concentration food sample: 25 microg/L). The complete duration of the method takes about 2 h.
Clarisse B S Roepcke; Susanne B Muench; Holger Schulze; Till T Bachmann; Till Bachmann; Rolf D Schmid; Bernhard Hauer
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-04     Completed Date:  2010-11-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8748-56     Citation Subset:  IM    
University of Stuttgart, Institute of Technical Biochemistry, Allmandring 31, 70569 Stuttgart, Germany.
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MeSH Terms
Acetylcholinesterase / analysis
Biosensing Techniques / methods*
Chloride Peroxidase / analysis
Enzymes, Immobilized / analysis
Food Contamination / analysis*
Organophosphorus Compounds / analysis*
Pesticides / analysis*
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Organophosphorus Compounds; 0/Pesticides; EC Peroxidase; EC
Erratum In:
J Agric Food Chem. 2010 Sep 22;58(18):10282
Note: Bachmann, Till [corrected to Bachmann, Till T]

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