| Analysis of PCR microchimerism induced by intrathymic inoculation of donor alloantigens in rats. | |
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MedLine Citation:
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PMID: 8889237 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intrathymic injection of donor alloantigens (splenocytes) was performed in rat heart transplantation to induced tolerance. In our experimental protocol, male (BN rats, RT1n) heart allografts were transplanted to female (LEW rats, RT1(1)) recipients in order to detect the existence of Y-chromosomes in recipients' tissues using the polymerase chain reaction (PCR) after the establishment of microchimerism. The mean survival time (MST) of male heart allografts was prolonged by the thymic injection protocol. PCR analysis of the Y-chromosomes of these recipients showed that splenocytes inoculated into the thymus still remained after as long as 30 days, while none of the cells that originated from the donor were never detected in the peripheral blood. On the other hand, our previous study demonstrated that the donor-specific Y-chromosomes could be detected in all the tissues of the females (peripheral blood, lymph nodes, spleen, and liver) except for thymus, of the sex-mismatched rat liver transplantation without thymic injection. Completely inconsistent results were deduced from two of our experiments, but the present study demonstrated that thymic clonal deletion played a major role in the prolongation of allograft survival after intrathymic injection of donor alloantigens. |
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Authors:
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M Furukawa; Y Fukuda; H Tashiro; H Ohdan; S Hoshino; S Shintaku; H Itou; D Kiyohiko |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cell transplantation Volume: 5 ISSN: 0963-6897 ISO Abbreviation: Cell Transplant Publication Date: 1996 Sep-Oct |
Date Detail:
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Created Date: 1997-01-31 Completed Date: 1997-01-31 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9208854 Medline TA: Cell Transplant Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: S75-7 Citation Subset: IM |
Affiliation:
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Second Department of Surgery, Hiroshima University School of Medicine, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Transplantation Chimera* DNA / analysis Female Graft Survival / drug effects, immunology Heart Transplantation / immunology*, mortality Immune Tolerance* Immunosuppressive Agents / pharmacology Isoantigens / pharmacology* Male Microinjections Polymerase Chain Reaction Rats Rats, Inbred BN Rats, Inbred Lew Sex Factors Spleen / cytology Survival Analysis Tacrolimus / pharmacology Thymus Gland / immunology* Tissue Donors Y Chromosome |
| Chemical | |
Reg. No./Substance:
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0/Immunosuppressive Agents; 0/Isoantigens; 109581-93-3/Tacrolimus; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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