Document Detail

Analysis of N-terminal processing of hepatitis C virus nonstructural protein 2.
MedLine Citation:
PMID:  8139048     Owner:  NLM     Status:  MEDLINE    
We determined the partial amino (N)-terminal amino acid sequence of hepatitis C virus p21 (nonstructural protein 2 [NS2]). Cleavage at the p21 (NS2) N terminus depended on the presence of microsomal membranes. The amino-terminal position of p21 (NS2) was assigned to amino acid 810 of the hepatitis C virus strain IIJ precursor polyprotein. Mutation of the alanine residue at position P1 of the putative cleavage site inhibited membrane-dependent processing. This alteration in processing together with the fact that hydrophobic amino acid residues are clustered upstream of the putative cleavage site suggested the involvement of a signal peptidase(s) in the cleavage. Furthermore, mutation analysis of this possible cleavage site revealed the presence of another microsome membrane-dependent cleavage site upstream of the N terminus of p21 (NS2).
H Mizushima; M Hijikata; Y Tanji; K Kimura; K Shimotohno
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  68     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-04-25     Completed Date:  1994-04-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2731-4     Citation Subset:  IM    
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Japan.
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MeSH Terms
Amino Acid Sequence
Base Sequence
DNA Mutational Analysis
Hepacivirus / metabolism*
Microsomes / metabolism
Molecular Sequence Data
Protein Processing, Post-Translational*
Recombinant Fusion Proteins / metabolism
Sequence Analysis
Tetrahydrofolate Dehydrogenase / biosynthesis,  genetics
Viral Nonstructural Proteins / genetics*
Reg. No./Substance:
0/Recombinant Fusion Proteins; 0/Viral Nonstructural Proteins; EC Dehydrogenase

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