Document Detail


Analysis of N-alkylated amino acids in human hemoglobin: evidence for elevated N-methylvaline levels in smokers.
MedLine Citation:
PMID:  7591184     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the contribution of cigarette smoking to the levels of N-methylvaline and N-hydroxyethylvaline in hemoglobin we analyzed samples from 32 smokers and 37 nonsmokers. The average background levels of the nonsmoking individuals were determined to be 1175 +/- 176 pmol N-methylvaline/g globin, ranging from 722 to 1516 pmol/g globin, and 46 +/- 12 pmol N-hydroxyethylvaline/g globin, ranging from 19 to 64 pmol/g globin. A significant correlation (P < 0.001) was found between both amino acids and the amount of cigarettes smoked per day, with an increase of 42 pmol N-methylvaline/g globin/cigarette per day and 11 pmol N-hydroxyethylvaline/g globin/cigarette per day. No influence of age, sex, and occupational exposure was observed. Furthermore, the levels of N-hydroxyethylvaline and N-methylvaline correlated for smokers but not for nonsmokers, indicating cigarette smoking as a common source for both adducts. To our knowledge, this is the first time N-methylvaline levels are reported to differ significantly between smokers and nonsmokers in the general population. Especially the analysis of N-methylvaline following low-level exposures to methylating agents should therefore take into consideration the influence of individual smoking habits. Additionally, the results of our study confirm the reliability of N-hydroxyethylvaline as an indicator of individual cigarette consumption. We successfully applied a new calibration technique to the analysis of N-hydroxyethylvaline, introducing a commercially available and well-defined dipeptide standard. The observed levels of N-hydroxyethylvaline in the samples are in line with those reported in the literature and verify the applicability of our calibration method.
Authors:
M Bader; J Lewalter; J Angerer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International archives of occupational and environmental health     Volume:  67     ISSN:  0340-0131     ISO Abbreviation:  Int Arch Occup Environ Health     Publication Date:  1995  
Date Detail:
Created Date:  1995-12-15     Completed Date:  1995-12-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7512134     Medline TA:  Int Arch Occup Environ Health     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  237-42     Citation Subset:  IM    
Affiliation:
Institute and Clinic of Occupational, Social and Environmental Medicine, University of Erlangen-Nümberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Calibration
Female
Hemoglobins / chemistry*
Humans
Male
Middle Aged
Reproducibility of Results
Smoking / blood*
Valine / analogs & derivatives*,  analysis
Chemical
Reg. No./Substance:
0/Hemoglobins; 7004-03-7/Valine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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