Document Detail

Analysis of N-acetyl and N-glycolylneuraminic acid in rat serum and tissues with Walker 256 carcinoma by high-performance liquid chromatography.
MedLine Citation:
PMID:  10191945     Owner:  NLM     Status:  MEDLINE    
Serum and tissue specimens from healthy Wistar rats and from rats with Walker 256 carcinoma were analysed for N-acetyl and N-glycolylneuraminic acid by high performance liquid chromatography (HPLC) as per-O-benzoylated derivatives. Both neuraminic acids were identified, while N-acetylneuraminic acid was the predominant sialic acid. Samples from rats with generalized metastasis showed a significant increase (45-80%) of total sialic acids. This phenomenon in serum is caused by the overproduction of sialic acids, as a result of synthesis of both types of neuraminic acids to a similar molar ratio. The increase of sialic acids in rat bones with metastatic cancer is mainly because of increased N-acetylneuraminic acid synthesis. These results suggest that the molecular mechanisms responsible for cancer metastasis in different tissues may be closely associated with increased synthesis of dominating neuraminic acid.
E Makatsori; A Aletras; N K Karamanos; T Tsegenidis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  13     ISSN:  0269-3879     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-06-07     Completed Date:  1999-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  57-60     Citation Subset:  IM    
Department of Chemistry, University of Patras, Greece.
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MeSH Terms
Carcinoma 256, Walker / blood,  metabolism*
Chromatography, High Pressure Liquid / methods*
N-Acetylneuraminic Acid / blood,  metabolism*
Neoplasm Transplantation
Neuraminic Acids / blood,  metabolism*
Rats, Wistar
Reg. No./Substance:
0/Neuraminic Acids; 1113-83-3/N-glycolylneuraminic acid; 131-48-6/N-Acetylneuraminic Acid

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