| Analysis of N-acetyl and N-glycolylneuraminic acid in rat serum and tissues with Walker 256 carcinoma by high-performance liquid chromatography. | |
| | |
MedLine Citation:
|
PMID: 10191945 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Serum and tissue specimens from healthy Wistar rats and from rats with Walker 256 carcinoma were analysed for N-acetyl and N-glycolylneuraminic acid by high performance liquid chromatography (HPLC) as per-O-benzoylated derivatives. Both neuraminic acids were identified, while N-acetylneuraminic acid was the predominant sialic acid. Samples from rats with generalized metastasis showed a significant increase (45-80%) of total sialic acids. This phenomenon in serum is caused by the overproduction of sialic acids, as a result of synthesis of both types of neuraminic acids to a similar molar ratio. The increase of sialic acids in rat bones with metastatic cancer is mainly because of increased N-acetylneuraminic acid synthesis. These results suggest that the molecular mechanisms responsible for cancer metastasis in different tissues may be closely associated with increased synthesis of dominating neuraminic acid. |
| | |
Authors:
|
E Makatsori; A Aletras; N K Karamanos; T Tsegenidis |
Related Documents
:
|
7057115 - Sialosylgalactosylceramide (gm4) is a major ganglioside in chicken embryonic liver. 6030295 - The linkage of sialic acid in the ehrlich ascites-carcinoma cell surface membrane. 6266645 - Neuraminidase activity and cell surface sialic acid turnover in rous sarcoma virus tran... 8070085 - Tamm-horsfall glycoprotein: role in inhibition and promotion of renal calcium oxalate s... 7118885 - Phospholipid biosynthesis in human platelets. formation of phosphatidylcholine from 1-a... 2750905 - A model study of the regulation of gastric acid secretion. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biomedical chromatography : BMC Volume: 13 ISSN: 0269-3879 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 1999 Feb |
Date Detail:
|
Created Date: 1999-06-07 Completed Date: 1999-06-07 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 57-60 Citation Subset: IM |
Affiliation:
|
Department of Chemistry, University of Patras, Greece. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Carcinoma 256, Walker / blood, metabolism* Chromatography, High Pressure Liquid / methods* N-Acetylneuraminic Acid / blood, metabolism* Neoplasm Transplantation Neuraminic Acids / blood, metabolism* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
|
0/Neuraminic Acids; 1113-83-3/N-glycolylneuraminic acid; 131-48-6/N-Acetylneuraminic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Direct injection HPLC analysis of some non-steroidal anti-inflammatory drugs on restricted access me...
Next Document: Direct injection of plasma to determine pseudoephedrine by high performance liquid chromatography wi...