| Analysis of mitochondrial pH and ion concentrations. | |
| | |
MedLine Citation:
|
PMID: 19957137 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Detailed practical information is provided with emphasis on mapping cytosolic and mitochondrial pH, mitochondrial Na(+), and briefly also aspects related to mitochondrial Ca(2+) measurements in living cells, as grown on (un)coated glass coverslips. This chapter lists (laser scanning confocal) microscope instrumentation and setup requirements for proper imaging conditions, cell holders, and an easy-to-use incubator stage. For the daily routine of preparing buffer and calibration solutions, extensive annotated protocols are provided. In addition, detailed measurement and image analysis protocols are given to routinely obtain optimum results with confidence, while avoiding a number of typical pitfalls. |
| | |
Authors:
|
Martin vandeVen; Corina Balut; Szilvia Baron; Ilse Smets; Paul Steels; Marcel Ameloot |
Related Documents
:
|
17543207 - An automatic system for the analysis of intercellular communication and early carcinoge... 9829777 - A computer-assisted system for reconstructing and interpreting the dynamic three-dimens... 9113307 - Comparative study of artificial neural networks in the discrimination between benign fr... 2695947 - Use of charge coupled device camera for imaging of intracellular phthalocyanines. 19012157 - In vivo brain imaging: fluorescence or bioluminescence, which to choose? 11276107 - A targeted contrast agent for magnetic resonance imaging of thrombus: implications of s... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 591 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
|
Created Date: 2009-12-03 Completed Date: 2010-02-18 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 275-309 Citation Subset: IM |
Affiliation:
|
Cell Physiology Group, Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Calcium / metabolism* Calibration Cell Line Cell Nucleus / metabolism Cell Survival Cytosol / metabolism Dogs Fluorescent Dyes / metabolism Glass Hydrogen-Ion Concentration Indicators and Reagents / metabolism Microscopy, Confocal Mitochondria / metabolism* Molecular Imaging Movement Sodium / metabolism* Software |
| Chemical | |
Reg. No./Substance:
|
0/Fluorescent Dyes; 0/Indicators and Reagents; 7440-23-5/Sodium; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Imaging of cellular oxygen and analysis of metabolic responses of Mammalian cells.
Next Document: Live cell imaging analysis of receptor function.