| Analysis of Legionella Infection by Flow Cytometry. | |
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MedLine Citation:
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PMID: 23150400 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Legionella pneumophila infects and replicates in environmental protozoa and metazoan macrophages within a specific vacuole. The infection of phagocytes by L. pneumophila can be assessed by an agar plating assay or by fluorescence microscopy. Here, we describe the analysis of Legionella infection by automated flow cytometry using wild-type and mutant bacteria that constitutively produce the green fluorescent protein (GFP). Advantages of the flow cytometry technique include (1) a software-assisted multiple parameter analysis of Legionella infections in real-time at distinct stages of the infection cycle, (2) the simultaneous and fast acquisition of a high number of data points, and (3) a characterization of the infecting bacteria in parallel with the infected host cells. |
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Authors:
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André N Tiaden; Aline Kessler; Hubert Hilbi |
Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 954 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: - |
Other Details:
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Languages: ENG Pagination: 233-249 Citation Subset: - |
Affiliation:
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Center for Applied Biotechnology and Molecular Medicine, University of Zurich, Zurich, Switzerland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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