Document Detail


Analysis of Legionella infection using RNAi in Drosophila cells.
MedLine Citation:
PMID:  23150401     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RNA interference (RNAi) is the process of specific gene silencing by the use of double-stranded RNA (dsRNA). In cultured Drosophila cells, RNAi methodologies are well established and easily executed: dsRNA, when added to the cell culture medium, is efficiently internalized by the cells and, through the activity of endogenous processing machinery, targets the specified mRNA for degradation resulting in reduced levels of its encoded protein. This technique has proven very useful in studying the role of host genes during Legionella pneumophila infections, as it allows the effect of host factor depletion on intracellular growth of the bacterium to be examined. In this chapter we present the methods commonly used in our laboratory to study intracellular growth of L. pneumophila using dsRNA in Drosophila cells.
Authors:
Dennise A De Jesús; Tamara J O'Connor; Ralph R Isberg
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  954     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-15     Completed Date:  2013-04-19     Revised Date:  2014-07-01    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  251-64     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Drosophila melanogaster / genetics*,  microbiology*
Gene Transfer Techniques
Legionella / physiology*
Legionellosis / genetics*,  microbiology*
RNA Interference*
RNA, Double-Stranded / chemical synthesis
RNA, Messenger / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
F31 AI098423/AI/NIAID NIH HHS; R37 AI023538/AI/NIAID NIH HHS; T32 GM007310/GM/NIGMS NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/RNA, Double-Stranded; 0/RNA, Messenger
Comments/Corrections

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