| Analysis of Hydrogenase 1 Levels Reveals an Intimate Link Between Carbon and Hydrogen Metabolism in Escherichia coli K-12. | |
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MedLine Citation:
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PMID: 22241049 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Two of the three [NiFe]-hydrogenases (Hyd) of Escherichia coli have a hydrogen-uptake function in anaerobic metabolism. While Hyd-2 is maximally synthesized when the bacterium grows by fumarate respiration, Hyd-1 synthesis shows a correlation with fermentation of sugar substrates. In an attempt to advance our knowledge on the physiological function of Hyd-1 during fermentative growth, we examined Hyd-1 activity and levels in various derivatives of E. coli K-12 MC4100 with specific defects in sugar utilisation. MC4100 lacks a functional fructose phosphotransferase system (PTS) and therefore grows more slowly under anaerobic conditions in rich medium in the presence of D-fructose compared to D-glucose. Growth in the presence of fructose resulted in an approximate 10-fold increase in Hyd-1 levels in comparison with growth under the same conditions with glucose. This increase in the amount of Hyd-1 was not due to regulation at the transcriptional level. Re-introduction of a functional fruBKA-encoded fructose PTS into MC4100 restored growth on D-fructose and reduced Hyd-1 levels to those observed after growth on D-glucose. Reducing the rate of glucose uptake by introducing a mutation in the gene encoding the cAMP receptor protein, or consumption through glycolysis, by introducing a mutation in phosphoglucose isomerase, increased Hyd-1 levels during growth on glucose. These results suggest that the ability to oxidize hydrogen by Hyd-1 shows a strong correlation with the rate of carbon flow through glycolysis and provides a direct link between hydrogen, carbon and energy metabolism. |
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Authors:
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Constanze Pinske; Jennifer S Macdowall; Frank Sargent; R Gary Sawers |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-12 |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: - ISSN: 1465-2080 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Martin-Luther University Halle-Wittenberg, Germany; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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