Document Detail


Analysis of Glycan-Related Genes Expression and Glycan Profiles in Mice with Liver Fibrosis.
MedLine Citation:
PMID:  23043565     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Protein glycosylation plays an important role in the pathogenesis and progression of various liver diseases. However, little is known about the precise alterations in protein glycosylation or the potential correlation between glycan-related genes expression and glycan profiles in liver fibrosis. The aim of the study was to investigate potential associations between glycan-related genes expression and glycan profiles to evaluate liver fibrosis in a mouse model. Analyses of glycan-related genes expression and glycan profiles were performed using oligonucleotide microarrays and lectin microarrays, respectively. Real-time PCR and Western blot were used to confirm any altered glycan-related genes expression levels and protein levels. Moreover, altered glycan patterns on the surface of hepatocytes were verified by lectin histochemistry. These results revealed that the mRNA levels of 10 glycan-related genes were significantly altered in fibrotic liver. Furthermore, we observed an increased in multivalent sialic acid, poly-LacNAc, sialyl-T-antigen, Fucoseα-1,3/6GlcNAc, and GalNAcα1-3Gal in fibrotic liver specimens, whereas GlcNAc oligomers was decreased in fibrotic liver. Our findings indicated that the synthetic pathway of "Tn antigen→ T antigen (core-1) → sialyl-T antigen" was activated for O-glycan during the process of liver fibrosis.
Authors:
Han-Jie Yu; Min-Zhi Zhu; Yan-Nan Qin; Yao-Gang Zhong; Hua Yan; Qi Wang; Hui-Jie Bian; Zheng Li
Related Documents :
8505265 - Status of research with transgenic farm animals.
8163675 - Phenotypic variability and incomplete penetrance of spontaneous fractures in an inbred ...
23471825 - An approach to transgene expression in liver endothelial cells using a liposome-based g...
15068235 - A new mouse model for down syndrome.
23235115 - Neuroprotective changes of thalamic degeneration-related gene expression by acupuncture...
21320115 - An association analysis at 2q36 reveals a new candidate susceptibility gene for juvenil...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-8
Journal Detail:
Title:  Journal of proteome research     Volume:  -     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hydrodynamic shear stress to remove Listeria monocytogenes biofilms from stainless steel and polytet...
Next Document:  Taiwanese long-term care facility residents' experiences of caring: a qualitative study.