| Analysis of gene expression profiles in fatal hepatic failure after hepatectomy in mice. | |
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MedLine Citation:
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PMID: 20444472 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: We developed 90%-hepatectomized mice that were the fatal model, and analyzed the gene expression profiles using a complementary DNA (cDNA) microarray to clarify the mechanisms of hepatic failure after excessive hepatectomy. MATERIALS AND METHODS: Ribonucleic acid (RNA)s from the remnant hepatic tissue of 70%- and 90%-hepatectomized mice were labeled with fluorescent dyes, and hybridized to the Riken set of 39,168 full-length enriched mouse cDNA arrays. The gene expression profiles in 90%- and 70%-hepatectomized mice were analyzed by scanning date for fluorescent dye signals. RESULTS: The down-regulated genes in 90%-hepatectomized mice were genes activating extracellular matrix (ECM) remodeling (matrix metalloproteinases, laminins, and integrins), genes related to cytokines (tumor necrosis factorα converting enzyme, and Janus kinase 3) that were related to the priming, genes related to growth factor (heparin-binding epidermal growth factor-like growth factor and others), and genes promoting cell cycle progression (cyclin D1, D2, and E2) that were related to the progression of hepatocytes. The up-regulated genes were genes inhibiting ECM remodeling [plasminogen activator inhibitors (PAIs)]. CONCLUSIONS: Hepatic failure after hepatectomy was characterized by the inhibition of hepatic cell cycle priming and progression both induced by ECM remodeling in liver regeneration. Particularly, the overexpression of PAIs was thought to play the major role in the first step of inhibition of ECM remodeling. |
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Authors:
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Hirochika Makino; Hiroshi Shimada; Daisuke Morioka; Chikara Kunisaki; Tomoyuki Morita; Ryusei Matsuyama; Toru Kubota; Daisuke Shimizu; Yasushi Ichikawa; Kuniya Tanaka; Kenichi Matsuo; Shinji Togo; Itaru Endo; Youji Nagashima; Yasushi Okazaki; Yoshihide Hayashizaki |
Publication Detail:
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Type: Journal Article Date: 2010-03-16 |
Journal Detail:
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Title: The Journal of surgical research Volume: 169 ISSN: 1095-8673 ISO Abbreviation: J. Surg. Res. Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-06-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: United States |
Other Details:
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Languages: eng Pagination: 36-43 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Gastroenterological Surgery, Yokohama City University School Graduate of Medicine, Yokohama, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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