Document Detail


Analysis of Flavonoids and Hydroxycinnamic Acid Derivatives in Rapeseeds (Brassica napus L. var. napus ) by HPLC-PDA-ESI(-)/MSn/HRMS.
MedLine Citation:
PMID:  24620834     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A comprehensive description of flavonoids and hydroxycinnamic acid derivatives in Brassica napus L. var. napus seeds is important to improve rapeseed quality. HPLC-PDA-ESI(-)/MSn/HRMS has been broadly applied to study phenolic compounds in plants. In the present study, crude phenolic compounds extracted from rapeseed were subjected to column chromatography, alkaline hydrolysis, and HPLC-PDA-ESI(-)/MSn/HRMS analysis. A total of 91 flavonoids and hydroxycinnamic acid derivatives were detected, including 39 kaempferol derivatives, 11 isorhamnetin derivatives, 5 quercetin derivatives, 6 flavanols and their oligomers, and 30 hydroxycinnamic acid derivatives. A total of 78 of these compounds were tentatively identified; of these, 55 are first reported in B. napus L. var. napus and 24 are first detected in genus Brassica. This research enriches our knowledge on the phenolic composition of rapeseed and provides a reliable guide for the selection of rapeseed with valuable breeding potential.
Authors:
Yanlin Shao; Jinjin Jiang; Liping Ran; Chunliang Lu; Cunxu Wei; Youping Wang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-3-12
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-3-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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