Document Detail


FLX pyrosequencing analysis of the effects of the brown-algal fermentable polysaccharides alginate and laminaran on rat cecal microbiotas.
MedLine Citation:
PMID:  23183985     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Edible brown algae are used as major food material in Far East Asian countries, particularly in South Korea and Japan. They contain fermentable dietary fibers, alginic acid (uronic acid polymer) and laminaran (β-1,3-glucan), that are fermented into organic acids by intestinal bacteria. To clarify the effect of edible algae on the intestinal environment, the cecal microbiotas of rats fed diets containing no dietary fiber (control) or 2% (wt/wt) sodium alginate or laminaran for 2 weeks were analyzed using FLX amplicon pyrosequencing with bar-coded primers targeting the bacterial 16S rRNA gene. The most abundant phylum in all groups was Firmicutes. Specifically, Allobaculum was dominant in all diet groups. In addition, Bacteroides capillosus (37.1%) was abundant in the alginate group, while Clostridium ramosum (3.14%) and Parabacteroides distasonis (1.36%) were only detected in the laminaran group. Furthermore, rats fed alginate showed simplified microbiota phylotypes compared with others. With respect to cecal chemical compounds, laminaran increased cecal organic acid levels, particularly propionic acid. Alginate increased total cecal organic acids. Cecal putrefactive compounds, such as indole, H(2)S, and phenol, were decreased by both alginate and laminaran. These results indicate that edible brown algae can alter the intestinal environment, with fermentation by intestinal microbiota.
Authors:
Choa An; Takashi Kuda; Takahiro Yazaki; Hajime Takahashi; Bon Kimura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-26
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  79     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-15     Completed Date:  2013-06-10     Revised Date:  2013-08-11    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  860-6     Citation Subset:  IM    
Affiliation:
Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alginates / metabolism*
Animals
Biodiversity*
Carboxylic Acids / analysis
Cecum / microbiology*
DNA Barcoding, Taxonomic
Diet / methods*
Glucuronic Acid / metabolism
Hexuronic Acids / metabolism
Metagenome*
Phaeophyta / chemistry*
Polysaccharides / metabolism*
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Rats
Chemical
Reg. No./Substance:
0/Alginates; 0/Carboxylic Acids; 0/Hexuronic Acids; 0/Polysaccharides; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid; 9008-22-4/laminaran
Comments/Corrections

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