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Analysis of ENPP2, a Lysophosphatidic Acid-Generating Enzyme, in the Uterus During Pregnancy in Pigs.
MedLine Citation:
PMID:  22914316     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Lysophosphatidic acid (LPA), a simple phospholipid, plays a critical role in the establishment of pregnancy in pigs. LPA production is mediated by ENPP2 action, a secreted lysophospholipase D (lysoPLD) that converts lysophosphatidylcholine to LPA. However, the regulatory mechanism of LPA production by ENPP2 in the porcine uterus is not well understood. In this study, we evaluated ENPP2 expression during the estrous cycle and pregnancy in the uterine endometrium and in early stage conceptuses. We also evaluated lysoPLD activity in the uterine lumen. ENPP2 transcripts and proteins were detected in the uterine endometrium at all stages of the estrous cycle and pregnancy with higher levels on day (D) 12 and D15 of the estrous cycle and pregnancy. ENPP2 expression was localized mainly to luminal and glandular epithelial cells in the endometrium, and was also detected in conceptuses on D12 of pregnancy. Secreted ENPP2 protein was detected in flushings of the uterine lumen on D12 of the estrous cycle and pregnancy, with higher levels on D12 of pregnancy. LysoPLD activity was detected in uterine flushings on D12 of the estrous cycle and pregnancy, with higher levels on D12 of pregnancy. This study showed that the uterine endometrium and conceptuses produce ENPP2 and secreted it into the uterine lumen where it has lysoPLD activity. These results suggest that ENPP2 may play an important role in the establishment of pregnancy in pigs by regulating LPA production at the maternal-conceptus interface.
Authors:
Heewon Seo; Yohan Choi; Jangsoo Shim; Mingoo Kim; Hakhyun Ka
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-22
Journal Detail:
Title:  Biology of reproduction     Volume:  -     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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