Document Detail


Analysis of DNA ploidy, cell cycle and Ki67 antigen in nasopharyngeal carcinoma by flow cytometry.
MedLine Citation:
PMID:  16116972     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The expression of DNA ploidy, the cell cycle and Ki67 antigen in nasopharyngeal carcinoma (NPC) were studied and their relationship with the clinical biological behaviors and prognosis of NPC was evaluated. Biopsied specimens of NPC were made into cell suspension. By using cytometric double labeling Ki67 and DNA method, the expression of DNA ploidy, the cell cycle and Ki67 antigen were analyzed. The patients were followed-up for about 3 years and the relationship between the above-mentioned parameters and the clinical biological behavior and prognosis of NPC were evaluated. Of the 62 cases of NPC, the DNA aneuploid accounted for 29.03%. The S phase cells accounted for 0 to 54% in the cell cycle and the positive expression of Ki67 ranged from 0 to 52%. There were 40 cases of LPI (64.5%) including 15 negative cases and 22 cases of HPI (35.5%) respectively. The DNA anueploid content was positively related to the S phase cells. The patients having a low expression of Ki67 or DNA aneuploid in tumor cells were not sensitive to chemotherapy, liable to metastasis to distant organs and had a poor prognosis, while Ki67 showed no correlation with DNA ploidy and the cell cycle. It was suggested that DNA ploidy and Ki67 could be used as an independent and objective marker to evaluate the radiosensitivity and prognosis of NPC.
Authors:
Xiaoyan Shi; Xianglin Yuan; Deding Tao; Jianping Gong; Guoqing Hu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban     Volume:  25     ISSN:  1672-0733     ISO Abbreviation:  J. Huazhong Univ. Sci. Technol. Med. Sci.     Publication Date:  2005  
Date Detail:
Created Date:  2005-08-24     Completed Date:  2006-05-30     Revised Date:  2006-12-14    
Medline Journal Info:
Nlm Unique ID:  101169627     Medline TA:  J Huazhong Univ Sci Technolog Med Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  198-201     Citation Subset:  IM    
Affiliation:
Oncological Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Carcinoma, Squamous Cell / immunology*,  metabolism,  pathology
DNA, Neoplasm / biosynthesis,  genetics*
Female
Humans
Ki-67 Antigen / immunology*
Male
Middle Aged
Nasopharyngeal Neoplasms / immunology*,  metabolism,  pathology
Ploidies
Prognosis
Tumor Cells, Cultured
Tumor Markers, Biological
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Ki-67 Antigen; 0/Tumor Markers, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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