| Analysis of the CtrA pathway in Magnetospirillum reveals an ancestral role in motility in alphaproteobacteria. | |
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MedLine Citation:
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PMID: 22467786 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Developmental events across the prokaryotic life cycle are highly regulated at the transcriptional and posttranslational levels. Key elements of a few regulatory networks are conserved among phylogenetic groups of bacteria, although the features controlled by these conserved systems are as diverse as the organisms encoding them. In this work, we probed the role of the CtrA regulatory network, conserved throughout the Alphaproteobacteria, in the magnetotactic bacterium Magnetospirillum magneticum strain AMB-1, which possesses unique intracellular organization and compartmentalization. While we have shown that CtrA in AMB-1 is not essential for viability, it is required for motility, and its putative phosphorylation state dictates the ability of CtrA to activate the flagellar biosynthesis gene cascade. Gene expression analysis of strains expressing active and inactive CtrA alleles points to the composition of the extended CtrA regulon, including both direct and indirect targets. These results, combined with a bioinformatic study of the AMB-1 genome, enabled the prediction of an AMB-1-specific CtrA binding site. Further, phylogenetic studies comparing CtrA sequences from alphaproteobacteria in which the role of CtrA has been experimentally examined reveal an ancestral role of CtrA in the regulation of motility and suggest that its essential functions in other alphaproteobacteria were acquired subsequently. |
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Authors:
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Shannon E Greene; Matteo Brilli; Emanuele G Biondi; Arash Komeili |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-03-30 |
Journal Detail:
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Title: Journal of bacteriology Volume: 194 ISSN: 1098-5530 ISO Abbreviation: J. Bacteriol. Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-05-14 Completed Date: 2012-08-06 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 2973-86 Citation Subset: IM |
Affiliation:
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Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GEO/GSE35625 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alphaproteobacteria
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classification,
cytology,
genetics,
metabolism* Amino Acid Sequence Bacterial Proteins / chemistry, genetics, metabolism* Binding Sites Biological Evolution Gene Expression Regulation, Bacterial Magnetospirillum / classification, cytology*, genetics, metabolism* Microbial Viability Molecular Sequence Data Phosphorylation Phylogeny Regulon Transcription Factors / chemistry, genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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GM07127/GM/NIGMS NIH HHS; R01 GM084122/GM/NIGMS NIH HHS; R01GM084122/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Transcription Factors |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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