Document Detail


Analysis of carbon metabolism and improvement of gamma-polyglutamic acid production from Bacillus subtilis NX-2.
MedLine Citation:
PMID:  19866376     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacillus subtilis NX-2 produces gamma-polyglutamic acid (gamma-PGA) when using glucose and L-glutamate as carbon sources. The conversion of carbon sources into gamma-PGA was analyzed with the (13)C-NMR method after enriching the media with (13)C-labeled glucose. The results showed that the percentage of gamma-PGA monomers derived from glucose was relatively low, approximately 6% and 9%, respectively, with an initial glucose concentration of 30 and 40 g L(-1). It was concluded that glucose was utilized mainly as the growth-limiting substrate for cell growth and supplied the required energy during gamma-PGA biosynthesis, while L-glutamate was preferred as the main substrate for gamma-PGA formation. To achieve an efficient conversion of L-glutamate and enhance the gamma-PGA production, a fed-batch culture was proposed by feeding of glucose. By this method, supplied L-glutamate (40 g L(-1)) was completely depleted, and gamma-PGA yield was attained 42 g L(-1).
Authors:
Jun Yao; Hong Xu; Ningning Shi; Xin Cao; Xiaohai Feng; Sha Li; Pingkai Ouyang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-29
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  160     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-18     Completed Date:  2010-06-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2332-41     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, School of Basic Medical Science, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Bacillus subtilis / metabolism*
Carbon / metabolism*
Glucose / metabolism
Glutamic Acid / metabolism
Isotope Labeling
Nuclear Magnetic Resonance, Biomolecular
Polyglutamic Acid / analogs & derivatives*,  metabolism
Chemical
Reg. No./Substance:
0/poly(gamma-glutamic acid); 25513-46-6/Polyglutamic Acid; 50-99-7/Glucose; 56-86-0/Glutamic Acid; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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