Document Detail

Analysis of carbon metabolism and improvement of gamma-polyglutamic acid production from Bacillus subtilis NX-2.
MedLine Citation:
PMID:  19866376     Owner:  NLM     Status:  MEDLINE    
Bacillus subtilis NX-2 produces gamma-polyglutamic acid (gamma-PGA) when using glucose and L-glutamate as carbon sources. The conversion of carbon sources into gamma-PGA was analyzed with the (13)C-NMR method after enriching the media with (13)C-labeled glucose. The results showed that the percentage of gamma-PGA monomers derived from glucose was relatively low, approximately 6% and 9%, respectively, with an initial glucose concentration of 30 and 40 g L(-1). It was concluded that glucose was utilized mainly as the growth-limiting substrate for cell growth and supplied the required energy during gamma-PGA biosynthesis, while L-glutamate was preferred as the main substrate for gamma-PGA formation. To achieve an efficient conversion of L-glutamate and enhance the gamma-PGA production, a fed-batch culture was proposed by feeding of glucose. By this method, supplied L-glutamate (40 g L(-1)) was completely depleted, and gamma-PGA yield was attained 42 g L(-1).
Jun Yao; Hong Xu; Ningning Shi; Xin Cao; Xiaohai Feng; Sha Li; Pingkai Ouyang
Related Documents :
18427736 - Fed-batch production of gluconic acid by terpene-treated aspergillus niger spores.
16668446 - Fluoride-induced inhibition of starch biosynthesis in developing potato, solanum tubero...
18561126 - Production of the macrolide antibiotic tylosin in cyclic fed-batch culture.
8439166 - Evidence for catabolite inhibition in regulation of pentose utilization and transport i...
23208166 - Diagnostic accuracy of an "amended" insulin-glucose ratio for the biochemical diagnosis...
15016156 - Single photon emission tomography (spect) study of regional cerebral blood flow in norm...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-29
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  160     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-18     Completed Date:  2010-06-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2332-41     Citation Subset:  IM    
Department of Biotechnology, School of Basic Medical Science, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacillus subtilis / metabolism*
Carbon / metabolism*
Glucose / metabolism
Glutamic Acid / metabolism
Isotope Labeling
Nuclear Magnetic Resonance, Biomolecular
Polyglutamic Acid / analogs & derivatives*,  metabolism
Reg. No./Substance:
0/poly(gamma-glutamic acid); 25513-46-6/Polyglutamic Acid; 50-99-7/Glucose; 56-86-0/Glutamic Acid; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A black mobile mass: diagnosis and management.
Next Document:  Toll-like receptors in pregnancy disorders and placental dysfunction.