Document Detail

Analysis of Biological Samples Using Paper Spray Mass Spectrometry: An Investigation of Impacts by the Substrates, Solvents and Elution Methods.
MedLine Citation:
PMID:  24072932     Owner:  NLM     Status:  Publisher    
Paper spray has been developed as a fast sampling ionization method for direct analysis of raw biological and chemical samples using mass spectrometry (MS). Quantitation of therapeutic drugs in blood samples at high accuracy has also been achieved using paper spray MS without traditional sample preparation or chromatographic separation. The paper spray ionization is a process integrated with a fast extraction of the analyte from the raw sample by a solvent, the transport of the extracted analytes on the paper, and a spray ionization at the tip of the paper substrate with a high voltage applied. In this study, the influence on the analytical performance by the solvent-substrate systems and the selection of the elution methods was investigated. The protein hemoglobin could be observed from fresh blood samples on silanized paper or from dried blood spots on silica-coated paper. The on-paper separation of the chemicals during the paper spray was characterized through the analysis of a mixture of the methyl violet 2B and methylene blue. The mode of applying the spray solvent was found to have a significant impact on the separation. The results in this study led to a better understanding of the analyte elution, on-paper separation, as well as the ionization processes of the paper spray. This study also help to establish a guideline for optimizing the analytical performance of paper spray for direct analysis of target analytes using mass spectrometry.
Yue Ren; He Wang; Jiangjiang Liu; Zhiping Zhang; Morgan N McLuckey; Zheng Ouyang
Publication Detail:
Journal Detail:
Title:  Chromatographia     Volume:  76     ISSN:  0009-5893     ISO Abbreviation:  Chromatographia     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0316520     Medline TA:  Chromatographia     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1339-1346     Citation Subset:  -    
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
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