| Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase. | |
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MedLine Citation:
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PMID: 12480114 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lipolytic system of Bacillus megaterium 370 was investigated, showing the existence of at least two secreted lipases and a cell-bound esterase. A gene coding for an extracellular lipase was isolated and cloned in Escherichia coli. The cloned enzyme displayed high activity on short to medium chain length (C(4)-C(8)) substrates, and poor activity on C(18) substrates. On the basis of amino acid sequence homology, the cloned lipase was classified into subfamily I.4 of bacterial lipases. |
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Authors:
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Cristian Ruiz; Ana Blanco; F I Javier Pastor; Pilar Diaz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEMS microbiology letters Volume: 217 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-12-13 Completed Date: 2003-03-28 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 263-7 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Federation of European Microbiological Societies |
Affiliation:
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Department of Microbiology, Faculty of Biology, University of Barcelona, Av Diagonal 645, 08028 Barcelona, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus megaterium
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enzymology*,
metabolism* Bacterial Proteins / genetics* Base Sequence Cloning, Molecular Electrophoresis, Polyacrylamide Gel Genes, Bacterial* Hot Temperature Hydrogen-Ion Concentration Lipase / genetics* Lipolysis |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/LipA protein, Bacteria; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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