Document Detail


Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase.
MedLine Citation:
PMID:  12480114     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lipolytic system of Bacillus megaterium 370 was investigated, showing the existence of at least two secreted lipases and a cell-bound esterase. A gene coding for an extracellular lipase was isolated and cloned in Escherichia coli. The cloned enzyme displayed high activity on short to medium chain length (C(4)-C(8)) substrates, and poor activity on C(18) substrates. On the basis of amino acid sequence homology, the cloned lipase was classified into subfamily I.4 of bacterial lipases.
Authors:
Cristian Ruiz; Ana Blanco; F I Javier Pastor; Pilar Diaz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  217     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-12-13     Completed Date:  2003-03-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  263-7     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Federation of European Microbiological Societies
Affiliation:
Department of Microbiology, Faculty of Biology, University of Barcelona, Av Diagonal 645, 08028 Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Bacillus megaterium / enzymology*,  metabolism*
Bacterial Proteins / genetics*
Base Sequence
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Genes, Bacterial*
Hot Temperature
Hydrogen-Ion Concentration
Lipase / genetics*
Lipolysis
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/LipA protein, Bacteria; EC 3.1.1.3/Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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