Document Detail


Analysis of B cell subsets following pancreatic islet cell transplantation in a patient with type 1 diabetes by cytometric fingerprinting.
MedLine Citation:
PMID:  20933515     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Manual gating of bivariate plots remains the most frequently used data analysis method in flow cytometry. However, gating is operator-dependent and cumbersome, particularly with the increasing complexity of modern multicolor immunophenotyping data. A method that can remove operator bias, enable systematic and thorough analysis of complex high-dimensional data, correlate temporal changes in different subsets and lead to biomarker discovery is needed. Here we apply such a method, called cytometric fingerprinting (CF), to data obtained on peripheral blood B cells from an adult patient with type-1 diabetes who underwent pancreatic islet transplantation. We establish that CF can be used to analyze longitudinal trends in immunophenotypic data, and show that results from CF are comparable to those obtained with traditional gating methods. Both methods reveal the appearance of transitional B cells and subsequent accumulation of more mature B cells following immunosuppression and transplantation. This pattern is consistent with a temporally ordered process of B cell auto-reconstitution. We also show the comparative efficiency of fingerprinting in recognizing relative changes in B cell subsets with respect to time, its ability to couple the data with statistical methods (agglomerative clustering) and its potential to define novel subsets.
Authors:
Debora R Sekiguchi; Jennifer A Sutter; Michael R Rickels; Ali Naji; Chengyang Liu; Kathleen J Propert; Wade T Rogers; Eline T Luning Prak
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-10-08
Journal Detail:
Title:  Journal of immunological methods     Volume:  363     ISSN:  1872-7905     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-17     Completed Date:  2011-01-24     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  233-44     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
B-Lymphocyte Subsets / immunology*
B-Lymphocytes / immunology*
Cluster Analysis
Diabetes Mellitus, Type 1 / immunology*,  surgery*
Female
Flow Cytometry / methods*
Humans
Immunophenotyping / methods*
Islets of Langerhans Transplantation / immunology*
Longitudinal Studies
Middle Aged
Prospective Studies
Grant Support
ID/Acronym/Agency:
T32 AR007442-23/AR/NIAMS NIH HHS; T32-AR-07442-23/AR/NIAMS NIH HHS; U01 DK070430-08/DK/NIDDK NIH HHS; U01-DK070430/DK/NIDDK NIH HHS
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