| Analysis of B cell subsets following pancreatic islet cell transplantation in a patient with type 1 diabetes by cytometric fingerprinting. | |
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MedLine Citation:
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PMID: 20933515 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Manual gating of bivariate plots remains the most frequently used data analysis method in flow cytometry. However, gating is operator-dependent and cumbersome, particularly with the increasing complexity of modern multicolor immunophenotyping data. A method that can remove operator bias, enable systematic and thorough analysis of complex high-dimensional data, correlate temporal changes in different subsets and lead to biomarker discovery is needed. Here we apply such a method, called cytometric fingerprinting (CF), to data obtained on peripheral blood B cells from an adult patient with type-1 diabetes who underwent pancreatic islet transplantation. We establish that CF can be used to analyze longitudinal trends in immunophenotypic data, and show that results from CF are comparable to those obtained with traditional gating methods. Both methods reveal the appearance of transitional B cells and subsequent accumulation of more mature B cells following immunosuppression and transplantation. This pattern is consistent with a temporally ordered process of B cell auto-reconstitution. We also show the comparative efficiency of fingerprinting in recognizing relative changes in B cell subsets with respect to time, its ability to couple the data with statistical methods (agglomerative clustering) and its potential to define novel subsets. |
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Authors:
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Debora R Sekiguchi; Jennifer A Sutter; Michael R Rickels; Ali Naji; Chengyang Liu; Kathleen J Propert; Wade T Rogers; Eline T Luning Prak |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-10-08 |
Journal Detail:
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Title: Journal of immunological methods Volume: 363 ISSN: 1872-7905 ISO Abbreviation: J. Immunol. Methods Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-17 Completed Date: 2011-01-24 Revised Date: 2012-01-09 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 233-44 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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B-Lymphocyte Subsets
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immunology* B-Lymphocytes / immunology* Cluster Analysis Diabetes Mellitus, Type 1 / immunology*, surgery* Female Flow Cytometry / methods* Humans Immunophenotyping / methods* Islets of Langerhans Transplantation / immunology* Longitudinal Studies Middle Aged Prospective Studies |
| Grant Support | |
ID/Acronym/Agency:
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T32 AR007442-23/AR/NIAMS NIH HHS; T32-AR-07442-23/AR/NIAMS NIH HHS; U01 DK070430-08/DK/NIDDK NIH HHS; U01-DK070430/DK/NIDDK NIH HHS |
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