Document Detail


Analysis of acyl fluxes through multiple pathways of triacylglycerol synthesis in developing soybean embryos.
MedLine Citation:
PMID:  19329563     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The reactions leading to triacylglycerol (TAG) synthesis in oilseeds have been well characterized. However, quantitative analyses of acyl group and glycerol backbone fluxes that comprise extraplastidic phospholipid and TAG synthesis, including acyl editing and phosphatidylcholine-diacylglycerol interconversion, are lacking. To investigate these fluxes, we rapidly labeled developing soybean (Glycine max) embryos with [(14)C]acetate and [(14)C]glycerol. Cultured intact embryos that mimic in planta growth were used. The initial kinetics of newly synthesized acyl chain and glycerol backbone incorporation into phosphatidylcholine (PC), 1,2-sn-diacylglycerol (DAG), and TAG were analyzed along with their initial labeled molecular species and positional distributions. Almost 60% of the newly synthesized fatty acids first enter glycerolipids through PC acyl editing, largely at the sn-2 position. This flux, mostly of oleate, was over three times the flux of nascent [(14)C]fatty acids incorporated into the sn-1 and sn-2 positions of DAG through glycerol-3-phosphate acylation. Furthermore, the total flux for PC acyl editing, which includes both nascent and preexisting fatty acids, was estimated to be 1.5 to 5 times the flux of fatty acid synthesis. Thus, recycled acyl groups (16:0, 18:1, 18:2, and 18:3) in the acyl-coenzyme A pool provide most of the acyl chains for de novo glycerol-3-phosphate acylation. Our results also show kinetically distinct DAG pools. DAG used for TAG synthesis is mostly derived from PC, whereas de novo synthesized DAG is mostly used for PC synthesis. In addition, two kinetically distinct sn-3 acylations of DAG were observed, providing TAG molecular species enriched in saturated or polyunsaturated fatty acids.
Authors:
Philip D Bates; Timothy P Durrett; John B Ohlrogge; Mike Pollard
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-03-27
Journal Detail:
Title:  Plant physiology     Volume:  150     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-04     Completed Date:  2009-07-23     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  55-72     Citation Subset:  IM    
Affiliation:
Departments of Biochemistry and Molecular Biology , Michigan State University, East Lansing, Michigan 48824-1312, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetates / analysis,  chemistry,  metabolism
Acylation
Carbon Radioisotopes
Diglycerides / chemistry,  metabolism
Fatty Acids / chemistry,  metabolism
Glycerol / analysis,  chemistry,  metabolism
Kinetics
Lipid Metabolism
Models, Biological
Phosphatidylcholines / chemistry,  metabolism
Seeds / growth & development,  metabolism*
Soybeans / embryology,  metabolism*
Triglycerides / biosynthesis*,  chemistry
Chemical
Reg. No./Substance:
0/Acetates; 0/Carbon Radioisotopes; 0/Diglycerides; 0/Fatty Acids; 0/Phosphatidylcholines; 0/Triglycerides; 56-81-5/Glycerol
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