Document Detail


Analyses of renal medullary lipid droplets from normal, hydronephrotic, and indomethacin treated rabbits.
MedLine Citation:
PMID:  1177669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipid droplets isolated from rabbit renal medullary tissue were analyzed and found to be composed of triglyceride and free fatty acids in a ratio of 2.9:1. These triglycerides were unique when compared to triglycerides of other rabbit tissues examined, in that they contained high percentages of octadecanoic acid (stearic acid, 9.8%), 5,8,11,14-eicosatetraenoic acid (arachidonic acid, 6.8%), and 7,10,13,16-docosatetraenoic acid (adrenic acid, 10%). Lipid droplet triglycerides were found to increase during experimental hydronephrosis and after administration of indomethacin, a prostaglandin synthetase and phosphodiesterase inhibitor. From gas liquid chromatography of fatty acid methyl esters of these triglycerides, it was determined that they were enriched further in their percent composition of 9,12-octadecadienoic acid (linoleic acid) and arachidonic acid, a prostaglandin precursor. The inverse relationship between lipid droplets and prostaglandin content in the inner medulla suggested a significant role of lipid droplet triglycerides as storage pools for prostaglandin precursors.
Authors:
K Comai; S J Farber; J R Paulsrud
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lipids     Volume:  10     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1975 Sep 
Date Detail:
Created Date:  1976-01-02     Completed Date:  1976-01-02     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  555-61     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cholesterol Esters / metabolism
Fatty Acids / metabolism
Hydronephrosis / metabolism*
Indomethacin / pharmacology*
Kidney / metabolism*
Kidney Cortex / metabolism
Kidney Medulla / drug effects,  metabolism*
Lipid Metabolism*
Male
Organ Specificity
Rabbits
Triglycerides / metabolism
Chemical
Reg. No./Substance:
0/Cholesterol Esters; 0/Fatty Acids; 0/Triglycerides; 53-86-1/Indomethacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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