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Anaerobic utilization of toluene by marine Alpha- and Gammaproteobacteria reducing nitrate.
MedLine Citation:
PMID:  23038808     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aromatic hydrocarbons are among the main constituents of crude oil and represent a major fraction of biogenic hydrocarbons. Anthropogenic influences as well as biological production leads to exposure and accumulation of these toxic chemicals in the water column and sediment of marine environments. The ability to degrade these compounds in situ has been demonstrated for oxygen- and sulphate-respiring marine microorganisms. However, if and to what extent nitrate-reducing bacteria contribute to the degradation of hydrocarbons in the marine environment and if these organisms are similar to their well-studied freshwater counterparts has not thoroughly been investigated. Here we determine the potential of marine prokaryotes from different sediments of the Atlantic Ocean and Mediterranean Sea to couple nitrate reduction to the oxidation aromatic hydrocarbons. Nitrate-dependent oxidation of toluene as electron donor in anoxic enrichment cultures was elucidated by analyses of nitrate, nitrite, and dinitrogen gas, accompanied by cell proliferation. The metabolically active members of the enriched communities were identified by RT-PCR of their 16S rRNA gene and subsequently quantified by fluorescent in situ hybridization. In all cases, toluene-grown communities were dominated by members of the Gammaproteobacteria, followed in some enrichments by metabolically active Alphaproteobacteria as well as members of the Bacteroidetes. From these enrichments, two novel denitrifying toluene-degrading strains belonging to the Gammaproteobacteria were isolated. Two additional toluene-degrading denitrifying strains were isolated from sediments from the Black Sea and the North Sea. These isolates belonged to the Alpha- and Gammaproteobacteria. Serial dilutions series with marine sediments indicated that up to 2.2x104 cells/cm3 were able to degrade hydrocarbons with nitrate as electron acceptor. These results demonstrated the hitherto unrecognized capacity of Alpha- and Gammaproteobacteria in marine sediments to oxidize toluene using nitrate.
Authors:
Karine Alain; Jens Harder; Friedrich Widdel; Karsten Zengler
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  -     ISSN:  1465-2080     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
CNRS, IUEM-UMR 6197, Laboratoire de Microbiologie des Environnements Extremes (LMEE);
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