Document Detail


Anabolic activity of ursolic acid in bone: Stimulating osteoblast differentiation in vitro and inducing new bone formation in vivo.
MedLine Citation:
PMID:  18822379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the field of osteoporosis, there has been growing interest in anabolic agents that enhance bone mass and improve bone architecture. In this study, we demonstrated that the ubiquitous plant triterpenoid, ursolic acid, enhances differentiation and mineralization of osteoblasts in vitro. We found that ursolic acid induced the expression of osteoblast-specific genes with the activation of mitogen-activated protein kinases, nuclear factor-kappaB, and activator protein-1. Additionally, noggin, an antagonist of bone morphogenetic proteins (BMPs), inhibited ursolic acid-induced osteoblast differentiation. Noggin also inhibited the activation of Smad and the induction of BMP-2 mRNA expression by ursolic acid in the late stage of osteoblast differentiation. Importantly, ursolic acid was shown to have bone-forming activity in vivo in a mouse calvarial bone formation model. A high proportion of positive immunostaining of BMP-2 was found in the nuclear region of woven bone formed by ursolic acid. These results suggested that ursolic acid has the anabolic potential to stimulate osteoblast differentiation and enhance new bone formation.
Authors:
Su-Ui Lee; Sang-Joon Park; Han Bok Kwak; Jaemin Oh; Yong Ki Min; Seong Hwan Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-07
Journal Detail:
Title:  Pharmacological research : the official journal of the Italian Pharmacological Society     Volume:  58     ISSN:  1043-6618     ISO Abbreviation:  Pharmacol. Res.     Publication Date:    2008 Nov-Dec
Date Detail:
Created Date:  2008-12-01     Completed Date:  2009-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907422     Medline TA:  Pharmacol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  290-6     Citation Subset:  IM    
Affiliation:
Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong-gu, Daejeon 305-600, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / genetics,  metabolism
Anabolic Agents*
Animals
Anthraquinones
Blotting, Western
Bone Development / drug effects*
Calcium / metabolism
Carrier Proteins / pharmacology
Cell Differentiation / drug effects*
Cell Survival / drug effects
Cells, Cultured
Coloring Agents
Genes, Reporter
Immunohistochemistry
Mice
Osteoblasts / drug effects*
RNA, Messenger / biosynthesis,  genetics
Skull / cytology,  drug effects
Triterpenes / pharmacology*
Chemical
Reg. No./Substance:
0/Anabolic Agents; 0/Anthraquinones; 0/Carrier Proteins; 0/Coloring Agents; 0/RNA, Messenger; 0/Triterpenes; 148294-77-3/noggin protein; 7440-70-2/Calcium; 77-52-1/ursolic acid; 83-61-4/Alizarin Red S; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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