Document Detail


AnDiaTec Salmonella sp. PCR-ELISA for analysis of food samples.
MedLine Citation:
PMID:  15330519     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A commercially available PCR kit (AnDiaTec Salmonella sp. PCR-ELISA) was developed and evaluated for the detection of Salmonella sp. in food samples. The test is based on PCR amplification and hybridization of the amplified DNA to a microtiter plate followed by the detection of PCR product in the manner of an enzyme-linked immunosorbent assay test. The sensitivity and specificity were evaluated first with Salmonella pure cultures and artificially contaminated food samples, including food types for which an inhibition of the PCR reaction was expected. Both experiments proved a very good sensitivity, specificity, and reliability of the test with a very broad range of food types. In a second evaluation study, more than 1,100 food samples of different types were tested in parallel with the PCR method and with the International Standardization Organization 6579 bacteriological reference method. The results of this evaluation study and the results from other experiments on dilutions of artificially contaminated food samples led to the establishment of a positive-negative cutoff value (optical density at 450 nm of more than 0.9) with respect to the conventional bacteriological method. Using this positive-negative cutoff, 98% agreement to the bacteriological method was obtained.
Authors:
Christoph Metzger-Boddien; Anja Bostel; Johannes Kehle
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  67     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-08-27     Completed Date:  2004-11-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1585-90     Citation Subset:  IM    
Affiliation:
AnDiaTec GmbH & Co KG, Leibnizstrasse 11, 70806 Kornwestheim, Germany.
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MeSH Terms
Descriptor/Qualifier:
DNA, Bacterial / analysis*
Enzyme-Linked Immunosorbent Assay / methods*
Evaluation Studies as Topic
Food Analysis
Food Contamination / analysis*
Food Microbiology*
Gene Amplification
Polymerase Chain Reaction / methods*
Reference Values
Reproducibility of Results
Salmonella / isolation & purification*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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