Document Detail


Amyloid-linked cellular toxicity triggered by bacterial inclusion bodies.
MedLine Citation:
PMID:  17307135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aggregation of proteins in the form of amyloid fibrils and plaques is the characteristic feature of some pathological conditions ranging from neurodegenerative disorders to systemic amyloidoses. The mechanisms by which the aggregation processes result in cell damage are under intense investigation but recent data indicate that prefibrillar aggregates are the most proximate mediators of toxicity rather than mature fibrils. Since it has been shown that prefibrillar forms of the nondisease-related misfolded proteins are highly toxic to cultured mammalian cells we have studied the cytoxicity associated to bacterial inclusion bodies that have been recently described as protein deposits presenting amyloid-like structures. We have proved that bacterial inclusion bodies composed by a misfolding-prone beta-galactosidase fusion protein are clearly toxic for mammalian cells but the beta-galactosidase wild type enzyme forming more structured thermal aggregates does not impair cell viability, despite it also binds and enter into the cells. These results are in the line that the most cytotoxic aggregates are early prefibrilar assemblies but discard the hypothesis that the membrane destabilization is the key event to subsequent disruption of cellular processes, such as ion balance, oxidative state and the eventually cell death.
Authors:
Nuria González-Montalbán; Antonio Villaverde; Anna Aris
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-09
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  355     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-06     Completed Date:  2007-05-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  637-42     Citation Subset:  IM    
Affiliation:
Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Amyloid / toxicity*
Animals
Cell Survival
Escherichia coli / genetics
Inclusion Bodies / chemistry*
Mice
NIH 3T3 Cells
Peptides / analysis,  genetics,  toxicity
Protein Folding
beta-Galactosidase / analysis*,  genetics,  toxicity*
Chemical
Reg. No./Substance:
0/Amyloid; 0/Peptides; EC 3.2.1.23/beta-Galactosidase

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