Document Detail


Amyloid beta 2-microglobulin is modified with imidazolone, a novel advanced glycation end product, in dialysis-related amyloidosis.
MedLine Citation:
PMID:  8995733     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have recently demonstrated by immunohistochemistry that amyloid beta 2-microglobulin (beta 2m) is modified with advanced glycation end products (AGEs) in dialysis-related amyloidosis (DRA). To further investigate the role of the Maillard reaction in the pathogenesis of DRA, we produced a monoclonal antibody to imidazolone, a novel AGE, and a reaction product of arginine and 3-deoxyglucosone (3-DG) which was accumulated in uremic serum. Then we determined the localization of imidazolone in the amyloid tissues by immunohistochemistry using the antibody. The connective tissues in carpal tunnel and ligamentum flavum were obtained from six patients with carpal tunnel syndrome and two patients with destructive spondyloarthropathy. Imidazolone was localized to all the beta 2m-positive amyloid deposits in these patients. Western blotting using the antibody demonstrated that beta 2m extracted from the synovium amyloid of hemodialysis patients was modified with imidazolone. Further, beta 2m isolated from the blood ultrafiltrate of hemodialyzed patients was also modified with imidazolone. In vitro incubation of beta 2m with 3-DG produced imidazolone-modified beta 2m. In conclusion, amyloid tissue beta2m is modified with imidazolone in patients with DRA. 3-DG accumulating in uremic serum may be involved in the modification of beta 2m with imidazolone.
Authors:
T Niwa; T Katsuzaki; S Miyazaki; T Momoi; T Akiba; T Miyazaki; K Nokura; F Hayase; N Tatemichi; Y Takei
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Kidney international     Volume:  51     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-03-31     Completed Date:  1997-03-31     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  187-94     Citation Subset:  IM    
Affiliation:
Nagoya University Branch Hospital, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aged
Amyloidosis / etiology,  metabolism*
Antibodies, Monoclonal
Antibody Specificity
Blotting, Western
Deoxyglucose / analogs & derivatives,  pharmacology
Enzyme-Linked Immunosorbent Assay
Female
Glycosylation End Products, Advanced / blood*,  chemistry,  immunology
Humans
Imidazoles / blood*,  chemistry,  immunology
Immunohistochemistry
Male
Middle Aged
Renal Dialysis / adverse effects*
beta 2-Microglobulin / analysis,  drug effects,  metabolism*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Glycosylation End Products, Advanced; 0/Imidazoles; 0/beta 2-Microglobulin; 154-17-6/Deoxyglucose; 2490-91-7/3-deoxyglucose

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