| Amylin improves the effect of leptin on insulin sensitivity in leptin-resistant diet-induced obese mice. | |
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MedLine Citation:
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PMID: 22275759 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Leptin enhances insulin sensitivity in addition to reducing food intake and body weight. Recently, amylin, a pancreatic β cell derived hormone, was shown to restore a weight-reducing effect of leptin in leptin-resistant diet-induced obesity (DIO). However, whether amylin improves the effect of leptin on insulin sensitivity in DIO is unclear. Diet-induced obesity (DIO) mice were infused with either saline (S), leptin (L, 500 μg/kg/day), amylin (A, 100 μg/kg/day), or leptin plus amylin (L/A) for 14 days using osmotic mini-pumps. Food intake, body weight, metabolic parameters, tissue triglyceride content and AMP-activated protein kinase (AMPK) activity were examined. Pair-feeding and weight-matched calorie restriction experiments were performed in order to assess the influence of food intake and body weight reduction. Continuous L/A coadministration significantly reduced food intake, increased energy expenditure and reduced body weight, while administration of L or A alone had no effects. L/A coadministration did not affect blood glucose levels during ad libitum feeding but significantly decreased plasma insulin levels (by 48%), suggesting the enhancement of insulin sensitivity. Insulin tolerance test actually showed the increased effect of insulin in L/A-treated mice. In addition, L/A coadministration significantly decreased tissue triglyceride content and increased α2 AMPK activity in skeletal muscle (by 67%). L/A coadministration enhanced insulin sensitivity more than pair-feeding and weight-matched calorie restriction. In conclusion, this study demonstrates the beneficial effect of L/A coadministration on glucose and lipid metabolism in DIO mice, indicating the possible clinical usefulness of leptin/amylin coadministration as a new anti-diabetic treatment in obesity-associated diabetes. |
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Authors:
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Toru Kusakabe; Ken Ebihara; Takeru Sakai; Licht Miyamoto; Daisuke Aotani; Yuji Yamamoto; Sachiko Yamamoto-Kataoka; Megumi Aizawa-Abe; Junji Fujikura; Kiminori Hosoda; Kazuwa Nakao |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-24 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: - ISSN: 1522-1555 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Kyoto University Graduate School of Medicine. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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