Document Detail

Amplified immunohistochemical detection of PrPsc in animal transmissible spongiform encephalopathies using streptomycin.
MedLine Citation:
PMID:  16549507     Owner:  NLM     Status:  MEDLINE    
Due to its sensitivity, immunohistochemistry (IHC) of abnormal prion protein (PrPsc) is used to study experimental and natural cases of transmissible spongiform encephalopathies (TSEs) such as Creutzfeldt-Jakob disease in humans or scrapie and bovine spongiform encephalopathy (BSE) in animals. The limits of detection are particularly critical when PrPsc IHC is used for diagnostic purposes. In this article, we describe for the first time the use of streptomycin sulfate in IHC, providing a novel original and easy way to amplify specifically PrPsc immunohistochemical detection in natural cases of BSE and scrapie, as well as in experimental TSEs in mice models using two different PrP antibodies.
Anna A Bencsik; Anthony W Coleman; Sabine O S Debeer; Hervé Perron; Aly Moussa
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Publication Detail:
Type:  Journal Article     Date:  2006-03-20
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  54     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-18     Completed Date:  2006-08-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  849-53     Citation Subset:  IM    
Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, Unité ATNC, Lyon, Cedex 07-France.
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MeSH Terms
Brain / metabolism
Encephalopathy, Bovine Spongiform / metabolism*
Immunohistochemistry / methods
Indicators and Reagents
Mice, Inbred C57BL
PrPC Proteins / metabolism*
Scrapie / metabolism*
Reg. No./Substance:
0/Indicators and Reagents; 0/PrPC Proteins; 57-92-1/Streptomycin

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