Document Detail


Amplified fragment length polymorphism (AFLP) is useful for finding markers associated with QTL for architectural trait in Hedysarum coronarium L.
MedLine Citation:
PMID:  17278708     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Amplified fragment length polymorphism (AFLP) has been proposed as a valuable tool for finding molecular markers linked to QTL controlling architectural trait. Segregation of polymorphic AFLP fragments was followed in F2 offsprings Hedysarum coronarium derived from two native wild accesions crossing: Jebel Zit x El Haouaria, which had previously characterised by morphological and AFLP markers. A comparison of profiles from the morphologically contrasted parental individuals and F2 offspring has been assessed using five AFLP primer combinations. These generated 178 bands revealed by silver-stained denaturing polyacrylamide gels, of which 150 were polymorphic. Several AFLP markers appeared to be implied in the orthotropic form that can assist the selection and improve Hedysarum forage crop.
Authors:
S Marghali; H Chennaoui; H Bourguiba; M Marrakchi; N Trifi-Farah
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta biologica Hungarica     Volume:  57     ISSN:  0236-5383     ISO Abbreviation:  Acta. Biol. Hung.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2007-02-06     Completed Date:  2007-03-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8404358     Medline TA:  Acta Biol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  459-71     Citation Subset:  IM    
Affiliation:
Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Campus universitaire, 2092 El Manar, Tunis, Tunisia.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Crosses, Genetic
DNA Primers / genetics
DNA, Plant / genetics
Fabaceae / genetics*,  growth & development,  physiology
Gravitropism / genetics
Nucleic Acid Amplification Techniques
Quantitative Trait Loci
Tunisia
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Plant

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