Document Detail

Amplified and In Situ Detection of Redox-Active Metabolite Using a Bio-based Redox-Capacitor.
MedLine Citation:
PMID:  23311878     Owner:  NLM     Status:  Publisher    
Redox-cycling provides a mechanism to amplify electrochemical signals for analyte detection. Previous studies have shown that diverse mediators/shuttles can engage in redox-cycling reactions with a bio-based redox-capacitor that is fabricated by grafting redox-active catechols onto a chitosan film. Here, we report that redox-cycling with this catechol-chitosan redox-capacitor can amplify electrochemical signals for detecting a redox-active bacterial metabolite. Specifically, we studied the redox-active bacterial metabolite pyocyanin that is reported to be a virulence factor and signalling molecule for the opportunistic pathogen, P. aeruginosa. We demonstrate that redox-cycling can amplify outputs from various electrochemical methods (cyclic voltammetry, chronocoulometry and differential pulse voltammetry) and can lower the detection limit of pyocyanin to 50 nM. Further, the compatibility of this bio-based redox-capacitor allows the in situ monitoring of the production of redox-active metabolites (e.g., pyocyanin) during the course of P. aeruginosa cultivation. We anticipate that the amplified output of redox-active virulence factors should permit an earlier detection of life-threatening infections by the opportunistic pathogen P. aeruginosa while the "bio-compatibility" of this measurement approach should facilitate in situ study of the spatiotemporal dynamics of bacterial redox signaling.
Eunkyoung Kim; Tanya Gordonov; William E Bentley; Gregory F Payne
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-13
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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