Document Detail

Amplification of the polyclonal activation of human T cells. I. Null-cell products promote the polyclonal proliferation of T cells.
MedLine Citation:
PMID:  6977479     Owner:  NLM     Status:  MEDLINE    
Synergy can be observed in the proliferative response to mitogens of cultures containing human T and Null cells when compared with those containing only highly purified cells of those two types. This synergy was analysed (i) by evaluation of the proliferative response at each step of the purification process leading to separation of T and Null cells; (ii) by back-mixing T and Nul cells at different rations; and (iii) by evaluation of the proliferative response of free suspension cultures of T cells overlaying a semi-solid layer containing Null cells, or of free suspension cultures of Null cells over a semi-solid culture layer of T cells. The following conclusions were reached: (i) purified Null cells are unresponsive to mitogen when cultured alone or in the presence of diffusible T-cell products; (ii) the T cells are less responsive when cultured alone than in the presence of Null cells or diffusible Null cell products. Thus the synergistic effect observed between T and Null cells is not due to the promotion of Null-cell proliferation by T -cell products but can be accounted for by diffusible Null-cell products enhancing the process of T lymphocyte activation by mitogens.
J Caraux; B Klein; C Thierry; B Serrou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  45     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  1982 Feb 
Date Detail:
Created Date:  1982-05-27     Completed Date:  1982-05-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  257-63     Citation Subset:  IM    
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MeSH Terms
Concanavalin A / pharmacology
Lymphocyte Activation*
Lymphocytes, Null / immunology*
Phytohemagglutinins / pharmacology
Pokeweed Mitogens / pharmacology
T-Lymphocytes / immunology*
Reg. No./Substance:
0/Phytohemagglutinins; 0/Pokeweed Mitogens; 11028-71-0/Concanavalin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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