Document Detail


Amplification of mitochondrial DNA fragments from ancient human teeth and bones
MedLine Citation:
PMID:  2113826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We extracted and visualized DNA from ancient human teeth and bones of 150 to 5,500 years B.P. from three deposits from the south of France. The DNA extracted was used as template for PCR with specific primers corresponding to a portion of the human mitochondrial genome. In our samples, we have amplified a specific DNA fragment of 121 bp which, in the case of one bone of 150 years B.P. has been cloned and sequenced. We show that this sequence is identical to the homologous region of human mitochondrial DNA. The striking implications of this new method for archaeological and paleontological studies are exposed.
Authors:
C Hänni; V Laudet; M Sakka; A Bègue; D Stéhelin
Publication Detail:
Type:  English Abstract; Historical Article; Journal Article    
Journal Detail:
Title:  Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie     Volume:  310     ISSN:  0764-4469     ISO Abbreviation:  C. R. Acad. Sci. III, Sci. Vie     Publication Date:  1990  
Date Detail:
Created Date:  1990-08-08     Completed Date:  1990-08-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8503078     Medline TA:  C R Acad Sci III     Country:  FRANCE    
Other Details:
Languages:  fre     Pagination:  365-70     Citation Subset:  IM; Q    
Affiliation:
Laboratoire de Morphologie évolutive, Muséum d'Histoire naturelle, Paris.
Vernacular Title:
Amplification de fragments d'ADN mitochondrial à partir de dents et d'os humains anciens.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Bone and Bones / ultrastructure*
DNA, Mitochondrial / isolation & purification*
Fossils
History, Ancient
Humans
Molecular Sequence Data
Nucleic Acid Amplification Techniques*
Paleodontology*
Polymerase Chain Reaction
Tooth / ultrastructure*
Chemical
Reg. No./Substance:
0/DNA, Mitochondrial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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