| Ampicillin Synthesis Using a Two-Enzyme Cascade with Both α-Amino Ester Hydrolase and Penicillin G Acylase. | |
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MedLine Citation:
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PMID: 22039394 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The current enzymatic production of semisynthetic β-lactam antibiotics requires isolation and purification of the intermediate 6-aminopenicillanic acid which adds cost and complexity to the manufacturing process. In this work, we took advantage of the unique substrate specificity of a-amino ester hydrolases to perform a purely aqueous one-pot production of ampicillin from penicillin G and D-phenylglycine methyl ester, catalyzed by α-amino ester hydrolase and penicillin G acylase. The synthesis was performed in both a one-pot, one-step synthesis resulting in a maximum conversion of 39%, and a one-pot, two-step process resulting in a maximum conversion of 47%. The two-enzyme cascade reported in this paper is a promising alternative to the current enzymatic two-step, two-pot manufacturing process for semisynthetic β-lactam antibiotics which requires intermittent isolation of 6-aminopenicillanic acid. |
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Authors:
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Janna K Blum; Andria L Deaguero; Carolina V Perez; Andreas S Bommarius |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: ChemCatChem Volume: 2 ISSN: 1867-3899 ISO Abbreviation: - Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2011-10-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101520610 Medline TA: ChemCatChem Country: - |
Other Details:
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Languages: ENG Pagination: 987-991 Citation Subset: - |
Affiliation:
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School of Chemical and Biomolecular Engineering Parker H. Petit Institute of Bioengineering and Bioscience 315 Ferst Drive, Atlanta, GA 30332-0363 (USA). |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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R01 AI064817-05//NIAID NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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