Document Detail


Amphiregulin promotes the proliferation of trophoblast cells during preimplantation development of porcine embryos.
MedLine Citation:
PMID:  19729194     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our objective was to investigate the effects of in vitro culture (IVC) medium supplemented with amphiregulin (AREG) on the preimplantation embryonic development of porcine (Genus: Sus domestica, Species: Landrace) embryos derived from in vitro fertilization (IVF) and parthenogenetic activation (PA). In vitro fertilization and PA embryos at the 1-cell stage were cultured in IVC medium supplemented with 0, 0.5, 5, or 50 ng/mL AREG for 7 d. There were significantly greater total numbers of cells in blastocysts of IVF and PA embryos cultured with 50 ng/mL AREG compared with that of controls. In vitro fertilization and PA embryos were then cultured in NCSU-23 medium supplemented with 50 ng/mL AREG on Days 1 through 7, Days 1 through 3 (early stage), or Days 4 through 7 (late stage), or without AREG. There were significantly greater numbers of trophoblast cells in the late-stage and full-term groups of IVF and PA embryos than in the early-stage and control groups. The presence of AREG protein in IVF-derived blastocysts was detected using a polyclonal AREG antibody and indirect immunofluorescence. Amphiregulin protein was localized in both the cytoplasm and nucleus. Using real-time polymerase chain reaction, we detected the expression of AREG mRNA in all developmental stages of IVF and PA embryos; however, the expression level varied according to stage. Thus, the incubation of porcine IVF and PA embryos in AREG-supplemented culture medium mainly at the late preimplantation stage increases the numbers of trophoblast cells.
Authors:
J H Lee; E Lee; D Biswas; C S Jeung; G S Lee; S H Hyun; E B Jeung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-03
Journal Detail:
Title:  Theriogenology     Volume:  72     ISSN:  1879-3231     ISO Abbreviation:  Theriogenology     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-05     Completed Date:  2010-01-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1023-31     Citation Subset:  IM    
Affiliation:
College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / metabolism
Culture Media
Cytoplasm / metabolism
Embryo Culture Techniques*
Embryonic Development / drug effects*
Fertilization in Vitro
Glycoproteins / analysis,  metabolism,  pharmacology*
Intercellular Signaling Peptides and Proteins / analysis,  metabolism,  pharmacology*
Polymerase Chain Reaction
RNA, Messenger / metabolism
Swine*
Trophoblasts / drug effects*,  metabolism
Chemical
Reg. No./Substance:
0/Culture Media; 0/Glycoproteins; 0/Intercellular Signaling Peptides and Proteins; 0/RNA, Messenger; 117147-70-3/amphiregulin

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