Document Detail

Amphiphilic helical peptide enhances the uptake of single-walled carbon nanotubes by living cells.
MedLine Citation:
PMID:  17895532     Owner:  NLM     Status:  MEDLINE    
The success of many projected applications of carbon nano-tubes (CNTs) to living cells, such as intracellular sensors and nanovectors, will depend on how many CNTs are taken up by cells. Here we report the enhanced uptake by HeLa cells of single-walled CNTs coated with a designed peptide termed nano-1. Atomic force microscopy showed that the dispersions were composed of individual and small bundles of nano-1 CNTs with 0.7- to 32-nm diameters and 100- to 400-nm lengths. Spectroscopic characterizations revealed that nano-1 disperses CNTs in a non-covalent fashion that preserves CNT optical properties. Elemental analyses indicated that our sample preparation protocol involving sonication and centrifugation effectively eliminated metal impurities associated with CNT manufacturing processes. We further showed that the purified CNT dispersions are taken up by HeLa cells in a time- and temperature-dependent fashion, and that they do not affect the HeLa cell growth rate, evidence that the CNTs inside cells are not toxic under these conditions. Finally, we discovered that approximately 6-fold more CNTs are taken up by cells in the presence of nano-1 compared with medium containing serum but no peptide. The fact that coating CNTs with a peptide enhances uptake offers a strategy for improving the performance of applications that require CNTs to be inside cells.
Shook-Fong Chin; Ray H Baughman; Alan B Dalton; Gregg R Dieckmann; Rockford K Draper; Carole Mikoryak; Inga H Musselman; Vasiliki Z Poenitzsch; Hui Xie; Paul Pantano
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  232     ISSN:  1535-3702     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-26     Completed Date:  2007-11-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1236-44     Citation Subset:  IM    
Department of Chemistry, The University of Texas at Dallas, Richardson, TX 75083-0688, USA.
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MeSH Terms
Cell Line
Hela Cells
Microscopy, Atomic Force
Nanotubes, Carbon / chemistry*
Peptides / chemistry*,  metabolism
Protein Structure, Secondary
Spectrum Analysis, Raman
Surface-Active Agents / metabolism
Time Factors
Reg. No./Substance:
0/Nano-1 peptide; 0/Nanotubes, Carbon; 0/Peptides; 0/Surface-Active Agents

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